Alkaloid compound, preparation method and application

[0051] The invention provides a kind of preparation method of alkaloid compound, specifically as follows:

[0052] S1, take a certain quality of Jiuzilian dry rhizome;

[0053]Use methanol or ethanol whose volume is 1 to 8 times the mass of the dried rhizome of Jiuzilian, heat and reflux for extraction 1 to 6 times near their respective boiling points, each time for 1 to 3 hours; wherein, if ethanol is used, the volume fraction of ethanol is 10 ~95%.

[0054] Alternatively, extract by cold soaking at room temperature for 1 to 5 times, each time for 1 to 3 hours;

[0055] Merge each extract and concentrate to obtain the total extract;

[0056] S2, the total extract obtained through step S1 is extracted at least once through ethyl acetate and n-butanol successively;

[0057] Liquid-liquid extraction is possible, that is, the total extract is suspended in water, the volume ratio of the total extract to water is 1:1 to 1:4, and the extract liquid is obtained, which is sequentia...

[0069] A kind of preparation method of alkaloid compound, concrete method is as follows:

[0070] 1) Take 20 kg of dried rhizomes of Jiuzilian, heat and reflux extract with methanol whose volume is 6 times the mass of dried rhizomes of Jiuzilian, extract 4 times, each time for 2 hours, combine the extracts to recover the solvent under reduced pressure, and obtain the total extract;

[0071] 2) Suspend the total extract in water of 4 times the amount, extract 4 times with ethyl acetate, then extract 4 times with n-butanol equal volume, and remove the organic solvent under reduced pressure in the extraction layer to obtain ethyl acetate and n-butyl alcohol respectively. Alcohol layer.

[0072] 3) Take 200 g of the n-butanol extraction layer, firstly, by using silica gel column chromatography, chloroform / methanol is 100:0~0:100 in volume ratio (v / v) for gradient elution, collect a fraction every 800mL, and pass through TLC A total of 15 fractions were obtained after identificati...

[0076] A kind of preparation method of alkaloid compound, concrete method is as follows:

[0077] 1) Take 20 kg of dried rhizomes of Jiuzilian, heat and reflux extraction with ethanol whose volume is 8 times the mass of dried rhizomes of Jiuzilian, and extract them under reflux for 3 times, each time for 3 hours, wherein the volume fraction of ethanol is 10%, and the combined extracts are decompressed Recover the solvent to obtain the total extract;

[0078] 2) Suspend the total extract in 3 times the amount of water, first extract 3 times with ethyl acetate, then extract 3 times with an equal volume of n-butanol, remove the organic solvent from the extraction layer to obtain ethyl acetate and n-butyl alcohol respectively Alcohol layer.

[0079] 3) Take 80 g of the n-butanol extraction layer, firstly, by using silica gel column chromatography, chloroform / methanol is 100:0~0:100 in volume ratio (v / v) for gradient elution, collect a fraction every 500mL, and pass through TLC A...