Saccharomycete cell wall breaking method

[0030] Example 1: Weigh 1.0 g of yeast, add reaction buffer to 5.0 mL, add 10 μL of toluene and mix for 2 min, shake at 37° C. and 120 rpm for 60 min to break the wall. That is, the toluene-broken enzyme liquid is obtained.

[0031] Example 2: Weigh 1.0 g of yeast, add reaction buffer to 5.0 mL, add 0.01 g / L complex enzyme and mix for 2 min, shake and break the wall at 37°C, pH 5.5, 120 rpm for 60 min. That is, the enzyme solution in which the composite enzyme is broken is obtained.

[0032] Wherein, the composition of each component in the compound enzyme is: 5% helicase, 70% lysozyme, 10% mannanase, and 15% dextranase.

[0033] Example 3: Weigh 1.0 g of yeast, add reaction buffer to 5.0 mL, add 0.02 g / L complex enzyme and mix for 2 min, shake and break the wall at 37°C, pH 5.5, 120 rpm for 60 min. That is, the enzyme solution in which the composite enzyme is broken is obtained.

[0034] Wherein, the composition of each component in the compound enzyme is: 5% helicase, 70% lysozyme, 10% mannanase, and 15% dextranase.

[0035] The enzyme activity after breaking the wall with toluene in Example 1 was calculated as 100%.

[0036] Yeast relative breaking rate (%)=(A / B)*100%

[0037] A: Compound Enzyme Breaking Fluid β-Galactosidase Enzyme Activity

[0038] B: β-galactosidase activity in toluene wall breaking solution

[0039] Table 1 Effect of 0.1% compound enzyme on yeast wall breaking

[0040]