Composite culture medium for producing chitosanase through fermentation

A technology of chitosanase and medium, applied in the field of microorganisms, can solve the problems of high cost of medium, large investment in equipment, fast speed, etc., and achieve the effects of improving fermentation level, no environmental impact, and low production cost

Active Publication Date: 2020-05-26
QINGDAO CREAT MEDIUM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common problem of physical degradation is that the rate of degradation is relatively fast in the early stage and slow in the later stage, which means that it takes a long time and high energy to degrade into smaller oligosaccharide molecules, and the effect is not good when used alone. The investment is relatively large, and there is no report on industrial production
[0004] However, there are deficiencies such as high medium cost and low enzyme fermentation ac

Method used

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  • Composite culture medium for producing chitosanase through fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Bacillus culture medium for the production of chitosanase by fermentation. 1000mL shake flask, the culture medium is composed of: specific molecular weight (8000-10000Da) chitosan 5g / L; yeast extract powder 12g / L; glucose 10g / L; inositol 0.01g / L; biotin 0.05mg / L; hydrochloric acid Thiamine 8g / L; riboflavin 3.5g / L; vitamin B12 4.2mg / L, pH 6.5. Seed solution was seeded for 24 hours, inoculated with 10%, filled with 10ml / 250mL, rotated at 175rpm / min, cultivated at 37°C for 48 hours, and the chitosanase activity reached 240U / ml.

Embodiment 2

[0016] Bacillus culture medium for the production of chitosanase by fermentation. 1000mL shake flask, the culture medium is composed of: specific molecular weight (8000-10000Da) chitosan 7.5g / L; yeast extract powder 10g / L; glucose 10g / L; inositol 0.03g / L; biotin 0.05mg / L; Thiamine hydrochloride 7g / L; riboflavin 4.0 / L; vitamin B12 3.5mg / L, pH value 6.5. Seed solution seed age is 24 hours, 10% inoculation, liquid volume 10ml / 250mL, rotating speed 200rpm / min, cultured at 37°C for 48 hours, and the chitosanase activity reaches 300U / ml.

Embodiment 3

[0018] Bacillus culture medium for the production of chitosanase by fermentation. 1000mL shake flask, the culture medium is composed of: specific molecular weight (8000-10000Da) chitosan 10g / L; yeast extract powder 8g / L; glucose 10g / L; inositol 0.01g / L; biotin 0.03mg / L; hydrochloric acid Thiamine 8g / L; Riboflavin 5.0 / L; Vitamin B12 3.5mg / L, pH 6.0. Seed solution seed age is 24 hours, 10% inoculation, liquid volume 10ml / 250mL, rotating speed 200rpm / min, cultured at 37°C for 48 hours, and the chitosanase activity reaches 210U / ml.

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Abstract

The invention relates to a composite culture medium for producing chitosanase through fermentation. The culture medium comprises chitosan with specific molecular weight, inositol, biotin, yeast extract powder, glucose, thiamine hydrochloride, riboflavin and vitamins. The culture medium disclosed by the invention can be used for bacillus to grow well and producing high-activity chitosanase. Compared with a common culture medium, the culture medium has the advantages that the yield of chitosanase is increased by 2-3 times and reaches 1.5 mg/mL, and the enzyme activity can reach 300,000 U/L. According to the culture medium, the production cost of chitosanase is greatly reduced, and the culture medium has extremely remarkable economic significance in application of chitosanase, especially in preparation of low-molecular-weight chitosan oligosaccharide by an enzyme method.

Description

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Claims

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Application Information

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Owner QINGDAO CREAT MEDIUM
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