Preparation method of dioleoyl phosphatidylcholine

[0031] (1) Add 50g glycerophosphorylcholine, 220g oleic acid, 95g DMAP, and 1000mL chloroform into a clean and dry 3000ml three-necked flask. Magnetically stir evenly. Dissolve 165 g of DCC in 1000 mL of chloroform, and quickly drop the DCC chloroform solution into the reaction system. Protected from light and reacted at 20°C for 12h. A large amount of white solid was produced, and the TLC detection reaction showed no further progress, so the reaction was stopped. Concentrate to dryness to obtain a yellow viscous liquid.

[0032] (2) Add solvent 1 acetone, beating and stirring for 3 hours, a large amount of solids are precipitated, and the crude product is obtained by drying. Heat the crude product with 1000ml of solvent 2 dichloromethane to 40°C and stir to dissolve, filter while hot to remove insoluble matter, cool the filtrate to 0°C, slowly add 1800mL of solvent 3 methyl tert-butyl ether dropwise, the system gradually becomes cloudy, slowly stirring has A large number of