Amino black staining solution, kit and method for detecting protein content

[0032] This embodiment provides a kit for detecting protein content, which includes amino black staining solution, rinsing solution, eluent and several sets of standard protein solutions with different concentrations. In addition, the kit may also include culture dishes, punches, nitrocellulose membranes, etc. required in the detection process, but is not limited thereto.

[0033] Among them, the amino black staining solution is obtained by adding 30mL of methanol, 10mL of glacial acetic acid, and 0.1g of amino black into the container, and distilling the volume to 100mL with water, and then mixing; several groups of standard protein solutions with different concentrations are Standard bovine serum albumin solution, its concentration is 2.5mg / mL, 1.25mg / mL, 0.625mg / mL, 0.313mg / mL, 0.156mg / mL, 0mg / mL; rinse solution is methanol water solution, its volume concentration is 50%; the eluent is sodium hydroxide solution with a molar concentration of 1mol / L.

[0035] This embodiment provides a kit for detecting protein content, which includes amino black staining solution, rinsing solution, eluent and several sets of standard protein solutions with different concentrations. In addition, the kit may also include culture dishes, punches, nitrocellulose membranes, etc. required in the detection process, but is not limited thereto.

[0036] Among them, the amino black staining solution is obtained by adding 20mL of methanol, 5mL of glacial acetic acid, and 0.05g of amino black into the container, and distilling the volume to 100mL with water, and then mixing; several groups of standard protein solutions with different concentrations are Standard bovine serum albumin solution, its concentration is 2.5mg / mL, 1.25mg / mL, 0.625mg / mL, 0.313mg / mL, 0.156mg / mL, 0mg / mL; rinse solution is methanol water solution, its volume concentration is 40%; the eluent is sodium hydroxide solution with a molar concentration of 0.5mol / L.

[0038] This embodiment provides a kit for detecting protein content, which includes amino black staining solution, rinsing solution, eluent and several sets of standard protein solutions with different concentrations. In addition, the kit may also include culture dishes, punches, nitrocellulose membranes, etc. required in the detection process, but is not limited thereto.

[0039] Among them, the amino black staining solution is obtained by adding 40mL of methanol, 15mL of glacial acetic acid, and 0.15g of amino black into the container, and distilling the volume to 100mL with water, and then mixing; several groups of standard protein solutions with different concentrations are Standard bovine serum albumin solution, its concentration is 2.5mg / mL, 1.25mg / mL, 0.625mg / mL, 0.313mg / mL, 0.156mg / mL, 0mg / mL; rinse solution is methanol water solution, its volume concentration is 60%; the eluent is sodium hydroxide solution with a molar concentration of 1.5mol / L.