Kit for jointly detecting three rheumatism items and preparation method of kit

The invention relates to the technical field of biology, and in particular, relates to a kit for jointly detecting three rheumatism items and a preparation method of the kit. The bottom of a clampingshell is provided with a bottom plate; an NC membrane is arranged in the middle of the bottom plate; a combination pad is arranged at the end part of the left side of the NC membrane; a sample pad ismounted on the left side of the combination pad; a water absorption pad is arranged at the end part of the left side of the NC membrane; an RF detection line, an ASO detection line, a CRP detection line and a quality control line are sequentially arranged on the NC membrane; a sample adding hole and an observation opening are formed in the upper surface of the clamping shell; the preparation process of the kit comprises the steps: carrying out proportioning reaction on a coating solution, a confining liquid, a microsphere resuspension and a combination pad treatment liquid, then carrying out microsphere activation and related latex treatment, and then carrying out related region spraying and other multi-step treatment to finally complete the preparation process. According to the technicalscheme, the kit for joint detection of three rheumatism items and the preparation method thereof can simultaneously detect multiple types of rheumatism items, are high in detection efficiency, simpleto operate and easy for result observation and identification.