Bacillus licheniformis for preventing and treating animal diarrhea and microecological preparation thereof

[0020] Bacillus, Bacillus, which prevention and treatment of animal diarrhea, the liquid medium of Bacillus subtilis: 10 ‰ Sodium chloride, 10 ‰ trypsin, 5 ‰ yeast powder, culture conditions: 37 ° C, PH Maintained at 7.4, Ventilation fermentation, obtaining the blend of Juji Bacillus; the solid medium is: 90% bran, 5% protein, 5% yeast powder, water is divided into 60%; culture conditions are: 37 ° C, ventilation fermentation, to obtain lichens Solid cultures of Bacillus Bacillus.

[0021] A microecological preparation for preventing diarrhea of ​​animal diarrhea, wherein the formulation contains the extract of the above-mentioned bacillus culture.

[0022] The preparation method of the extract is to cultivate the bacillus solid culture for 5 days at 32 ° C for 24 hours, and then the carrier is adsorbed and dried at a low temperature.

[0023] The carrier includes one or several of wheat bran, corn core, yeast cell wall, corn starch, rice bran, glucose, zeolite powder, bentonite, mon

[0028] Example 2 Prevention and treatment capabilities of Bacillus undergarment

[0029] First, test animals and test diet

[0030] The strains of the purified strain purified in Example 1 were physically activated, and 25HR was activated to obtain a culture solution of the strain of the present invention. The test was selected from 100 heads of the year, with an average weight of 7.37 ± 0.02 kg. 1 As a blank control group, feeding the base diet. 2 As a test group, feeding the test day grain, adding the amount of Bacillus 200ml / t under the basis of the base diet, of which 0 billion CFU / g of Bacillus Ground Bacillus. The test is recorded in the whole process of each group of feed diarrhea, which weighed on the first day of the test, and the surplus feed amount was weighed, and the amount of the surplus feed was calculated, and the average daily weight loss, the meat ratio (F / g) was calculated.

[0031] Second, data analysis

[0032] Single factor analysis and correlation a

[0039] Example 3: Bacillus undergarment to Golden Staphylococcus, Pig Escherichia coli, Salmonella

[0040] First, test samples and treatment

[0041] Example 1 The purified strain was carried out to activate liquid LB culture, activated 36HR and obtained a culture solution of the strain of the present invention. Pig Escherichia coli, Golden Staphylococcus, Salmonella in a liquid LB medium. Liquid LB medium formula is 1%, NaCl 1%, 0.5% yeast powder, 1.5%, pH 7.4.