Particle size variable anti-tumor bionic nano preparation based on PLGA and preparation method and application thereof

[0038] A PLGA-based variable particle size anti-tumor biomimetic nano-preparation provided by the present invention, the biomimetic nano-preparation includes PLGA, bufatalin (CS-6), PEGylated graphene oxide quantum modified by photosensitizer chlorin e6 Dots (GC) and biomimetic hybrid membrane (HM), photosensitizer chlorin e6 (Ce6) modified on the surface of PEGylated graphene oxide quantum dots, bufatalin (CS-6) and chlorin modified PEGylated graphene oxide quantum dots (GC) were embedded in PLGA to synthesize nanoformulations (GC&CS-6@PLGA), and biomimetic hybrid membrane (HM) was camouflaged in the outer layer of nanoformulations (GC&CS-6@PLGA) to synthesize biomimetic Nano-preparation (GC&CS-6@PLGA[HM]), the particle size of biomimetic nano-preparation is 100nm~130nm.

[0039] The invention provides a method for preparing the above-mentioned PLGA-based variable particle size anti-tumor biomimetic nano-preparation, comprising the following steps:

[0040] S1. Take the whole b

[0052] The application of a PLGA-based anti-tumor biomimetic nano-preparation with variable particle size in light / chemotherapy combined tumor treatment uses the GC&CS-6@PLGA[HM] biomimetic nano-preparation prepared in Example 1.

[0053] (1) Study on the permeability of 3D balls

[0054] The in vitro tumor 3D spherical model was used to measure the cell penetration ability of the GC&CS-6@PLGA[HM] biomimetic nano-preparation prepared in Example 1 with or without laser irradiation in a slightly acidic environment. The light wavelength was 660nm, and the light energy density was 100mW / cm2, CS-6 drug concentration is 40ng / mL, photosensitizer Ce6 concentration is 5μg / mL, the experimental results are as follows Figure 4 As shown, the small particle size material GC has good penetration ability compared with Ce6, which shows that the small particle size material has advantages in penetration; in addition, the PLGA biomimetic nano-preparation co-loaded with CS-6 and GC has goo

[0060] Using the biomimetic nano-preparation GC&CS-6@PLGA[HM] prepared in Example 1, the blood half-life and biodistribution of the biomimetic nano-preparation were measured by semi-quantitative means of detecting the fluorescence intensity.

[0061] After tail vein injection of 100 μL of Ce6, GC, GC&CS-6@PLGA or GC&CS-6@PLGA[HM] at a dose concentration of 5 mg / kg, blood samples were collected at different time points for fluorescence intensity measurement, and the results were as follows: Image 6 As shown, the blood circulation half-lives of Ce6, GC, GC&CS-6@PLGA and GC&CS-6@PLGA[HM] were 1.5±0.1h, 2.0±0.2h, 3.1±0.3h and 4.5±0.4h, respectively. Compared with Ce6, GC and GC&CS-6@PLGA, the blood circulation period of GC&CS-6@PLGA[HM] was significantly prolonged, 1.3 times that of GC&CS-6@PLGA and 2.3 times that of GC. It shows that the nanomaterials camouflaged by the biomimetic hybrid membrane are beneficial to prolong the blood circulation time. at the same time, Image 6 T