Application of bioactive peptide in inhibition of bone marrow toxicity

[0017] Example a: Albumin peptides and oligopeptides Inhibition Corn cisplatin (DDP) side effects of

[0018] 1. Experimental Animals

[0019] 6 to 8 week old SPF grade male C57BL / 6 mice were purchased from the Experimental Animal Center of Henan (Production License: SCXK (Yu) 2017-0001), for feeding to the Laboratory Animal Room liver pharmacologically Henan. After adaptive feeding and observed for 5 days for health active selected mice experiments.

[0020] 2. Experimental methods

[0021] 2.1 Grouping and administration

[0022] The 24 weighing 18 ~ 20g SPF level of C57BL / 6 mice were randomly divided into four groups, A: control group, B: DDP group, C: DDP + ovalbumin peptide group, D: DDP + Y corn oligopeptide Group. B, C, D group in daily 4mg / kg dosage by intraperitoneal injection DDP, 5 days, A group were intraperitoneally injected normal saline. After 1 hour, C, and D, respectively, and then 600mg / kg (0.2mL / 10g) and ovalbumin peptide gavage dose of corn oli

[0033] Example Two: Corn and ovalbumin peptide inhibition studies oligopeptides of doxorubicin-induced toxicity (of DOX)

[0034] 1. Experimental Animals

[0035] 6 to 8 week old SPF grade male C57BL / 6 mice were purchased from the Experimental Animal Center of Henan (Production License: SCXK (Yu) 2017-0001), for feeding to the Laboratory Animal Room liver pharmacologically Henan. After adaptive feeding and observed for 5 days for health active selected mice experiments.

[0036] 2. Experimental methods

[0037] 2.1 Grouping and administration

[0038] The 24 weighing 18 ~ 20g SPF level of C57BL / 6 mice were randomly divided into four groups, A: control group, B: DOX group, C: DOX + ovalbumin peptide group, D: DOX + Corn oligopeptide group . B, C, D group to 17mg / kg intraperitoneal dose of DOX, A group were intraperitoneally injected normal saline. After 1 hour, C, and D, respectively, and then 600mg / kg (0.2mL / 10g) and ovalbumin peptide gavage dose of corn oligopept

[0049] Example III: ovalbumin peptide inhibition studies and corn oligopeptides toxicity induced by daunorubicin (DNR)

[0050] 1. Experimental Animals

[0051] 6 to 8 week old SPF grade male C57BL / 6 mice were purchased from the Experimental Animal Center of Henan (Production License: SCXK (Yu) 2017-0001), for feeding to the Laboratory Animal Room liver pharmacologically Henan. After adaptive feeding and observed for 5 days for health active selected mice experiments.

[0052] 2. Experimental method

[0053] 2.1 Grouping and administration

[0054] SPF grade C57BL / 6 mice with 24 g weight of 18 to 22 g were randomly divided into 4 groups, A: Control group, B: Softxamicin (DNR) group, C: DNR + albin peptide group, D: DNR + Corn low polypeptide group. B, C, and D group injected DNR in a 1 mg / kg dose, a group of abdominal injection isometric normal saline. After 1 hour, C, D group were given a 600 mg / kg (0.2 ml / 10g) dose intravascular polyprotin peptide and corn oligope