Fluorescent nano-particle with surface biological function, its production and use

[0041] Preparation Method 1 of Core-shell Fluorescent Nanoparticles

[0042] Mix isopropanol and water uniformly at a ratio of 5:1, and sonicate for 5 minutes. Weigh 0.1-0.8 mg of fluorescent substance fluorescein isothiocyanate FITC, configure it into an aqueous solution, and process it ultrasonically for 10 min. Pour the upper clear layer into a three-necked flask, and keep stirring to keep it in a dispersed state. Take 1mL of concentrated ammonia water and slowly add it into the constantly stirring solution system. Then take 1-3 mL of tetraethyl orthosilicate, and also slowly add it into the constantly stirring solution system. The system was reacted at room temperature for 3 to 5 hours. The reaction product was poured out, and the particles were washed 3 to 5 times with twice distilled water. The cleaned particles were vacuum-dried for 5 hours at a temperature of 20-50° C., and the particles were collected for future use. From figure 1 We can see that the silica has ...

[0044] Preparation Method 2 of Core-shell Fluorescent Nanoparticles

[0045] Mix TritonX-100, n-hexanol and cyclohexane uniformly at a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the above-mentioned microemulsion system in ultrasonic treatment for 30-60 minutes, add 0.5 mg of fluorescent substance fluorescein isothiocyanate FITC to it, use ultrasonic treatment for 6 minutes, take out the supernatant liquid and pour it into a three-necked flask, stir for 30 minutes to make It's even. Take 1mL of concentrated ammonia water and dilute it with 2mL double-distilled water, slowly add it into the microemulsion under constant stirring after 30 minutes, and keep stirring for 30 minutes to disperse the ammonia water evenly in the microemulsion. After 1 hour, 1-3 mL of tetraethyl orthosilicate was added dropwise to the microemulsion, while stirring continuously for 10 hours, and the temperature of the system was kept between 15-30°C. Add acetone to the sys...

[0047] Amino modification on the surface of fluorescent nanoparticles

[0048] Take 20 mg of the fluorescent nanoparticles prepared in Example 1 or 2, add 30-50 mL of methanol and glycerol in a 5:3 mixture, and use ultrasonic treatment for 20-60 minutes; weigh 1-3 mL of AEAPS [N-(2-aminoethyl)-3-aminopropyltrimethoxysilane], ultrasonic treatment for 10 to 60 minutes; mix the two solutions evenly, react at 60°C for 5 hours, and then take out The particles were washed with methanol for 3 times, followed by vacuum drying at 40-80° C. for 2 hours, and the particles were collected to obtain biofunctionalized fluorescent nanoparticles with amino groups modified on the surface.