The embodiment of the invention belongs to the technical field of detection, and relates to a time-resolved fluorescence immunochromatography kit for quantitatively detecting NT-proBNP/ST2 and application of the time-resolved fluorescence immunochromatography kit, the kit comprises a detection card, the detection card comprises a card shell and a test strip, the test strip comprises a PVC bottom plate, and the PVC bottom plate is provided with a card cover. A treatment pad, a combination pad, a nitrocellulose membrane and absorbent paper which are in lap joint in sequence are arranged on the PVC bottom plate; wherein a fluorescently-labeled NT-proBNP-L antibody, a fluorescently-labeled ST2-L antibody and a fluorescently-labeled goat anti-chicken IgY antibody are fixed on the conjugate pad, a first detection line, a second detection line and a quality control line are sequentially arranged on the nitrocellulose membrane based on the direction from the conjugate pad to the absorbent paper, the first detection line is coated with the NT-proBNP-C antibody, the second detection line is coated with the goat anti-chicken IgY antibody, and the quality control line is coated with the goat anti-chicken IgY antibody. The second detection line is coated with an ST2-C antibody, and the quality control line is coated with a chicken IgY antibody. According to the application, the values of the amino-terminal brain natriuretic peptide precursor and the growth stimulation expression gene 2 protein in the heart failure condition can be rapidly detected.