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3 results about "Amino terminal" patented technology

Amino-terminal (not comparable) (biochemistry) Describing the amino acid, at one end of a polypeptide or protein, that has a free amino group.

Kit for quantitatively detecting NT-proBNP/ST2 by time-resolved fluorescence immunochromatography and application thereof

PendingCN114167052ASimple and fast operationQuick checkBiological testingGenes proteinsExpression gene
The embodiment of the invention belongs to the technical field of detection, and relates to a time-resolved fluorescence immunochromatography kit for quantitatively detecting NT-proBNP/ST2 and application of the time-resolved fluorescence immunochromatography kit, the kit comprises a detection card, the detection card comprises a card shell and a test strip, the test strip comprises a PVC bottom plate, and the PVC bottom plate is provided with a card cover. A treatment pad, a combination pad, a nitrocellulose membrane and absorbent paper which are in lap joint in sequence are arranged on the PVC bottom plate; wherein a fluorescently-labeled NT-proBNP-L antibody, a fluorescently-labeled ST2-L antibody and a fluorescently-labeled goat anti-chicken IgY antibody are fixed on the conjugate pad, a first detection line, a second detection line and a quality control line are sequentially arranged on the nitrocellulose membrane based on the direction from the conjugate pad to the absorbent paper, the first detection line is coated with the NT-proBNP-C antibody, the second detection line is coated with the goat anti-chicken IgY antibody, and the quality control line is coated with the goat anti-chicken IgY antibody. The second detection line is coated with an ST2-C antibody, and the quality control line is coated with a chicken IgY antibody. According to the application, the values of the amino-terminal brain natriuretic peptide precursor and the growth stimulation expression gene 2 protein in the heart failure condition can be rapidly detected.
Owner:广州达泰生物工程技术有限公司

Liver targeted peptide-recombinant human endostatin fusion protein, and preparation method and application thereof

ActiveCN103319606AStrong specificityImprove the dose-effect ratioBacteriaPeptide/protein ingredientsSide effectWhole body
The invention discloses a liver targeted peptide-recombinant human endostatin fusion protein, and a preparation method and application thereof. According to the invention, 19 amino acids in the N terminal conserved I region of plasmodium circumsporozoite protein (CSP) capable of being specifically combined with hepatocyte surface receptor--heparan sulfate proteoglycan (HSPG) are fused to the carboxyl terminal of a novel human endostatin (nhES), the amino terminal of which is added with 9 amino acid sequences (MGGSHHHHH), by a gene engineering method, thereby obtaining the liver targeted peptide-recombinant human endostatin fusion protein (ES-CPS). The fusion protein is easy to purify, can be specifically used in livers for inhibiting neovascularization in a targeted mode, enhances the local concentration of the medicine in the foci, reduces the consumption to the whole body, and lowers the toxic or side effect. The invention provides an idea and scientific reference for clinically developing medicines for treating liver cancers by targeted inhibition of neovascularization, and has huge scientific meanings and practical value for further enhancing the liver cancer treatment level in China.
Owner:GUANGDONG PHARMA UNIV
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