9 results about "Hepatocyte" patented technology

The invention provides methods and media for culturing embryonic stem (ES) cells, such as human ES cells, and directing them along the hepatic lineage. It further provides methods for maintaining hepatocytes in culture for extended periods. The invention further provides cells cultured by the methods of the invention. Additionally, the invention provides methods of transducing cells with marker proteins that will be expressed only in hepatocyte-like cells and selecting for cells expressing the marker protein.

The invention relates to a method for efficiently amplifying subgroup J avian leukosis virus (ALV). According to the method, chicken hepatocytes can be used for efficiently amplifying the subgroup J ALV, the characteristic of high titer virus can be produced earlier by using the chicken hepatocytes, the separation and detection time of the subgroup J ALV can be shortened, the pathogen can be detected earlier, and the sensitivity of a subgroup J ALV detection method is improved. When subgroup J ALV infection MOI is 0.001 hour, the effect of enzyme-linked immunosorbent assay (ELISA) on the chickhepatocytes infected for 3 days is comparable to the effect of ELISA on DF-1 cells infected for 7 days. The efficient amplification method established by the invention accelerates the purification process of the subgroup J ALV, thus shortening the time and reducing cost; the method has very good application value in the purification detection of the subgroup J ALV.

The invention relates to dendrobe and turtle healthcare soup which is prepared from turtles, a dendrobe product, black beans, red dates, ginger and kelp by using processes of enzymolysis, extraction and mixing. The dendrobe and turtle healthcare soup provided by the invention is applicable to patients suffering from hyperlipemia, coronary heart diseases, hepatitis and hepatosplenomegaly, can promote blood circulation, promote qi circulation and remove obstruction, can prevent platelet aggregation, reduce blood fat and cholesterol deposition, treat and relieve hyperlipidemia and coronary heart diseases and reduce the morbidity, can also remove in-vivo inflammation and remedy damaged hepatocyte, and has relatively good relieving and treatment effects on hepatitis symptoms.

Alcohol intoxication causes serious diseases, whereas current treatments are mostly supportive and unable to remove alcohol efficiently. Upon alcohol consumption, alcohol is sequentially oxidized to acetaldehyde and acetate by the endogenous alcohol dehydrogenase and aldehyde dehydrogenase, respectively. We disclose a hepatocyte-mimicking antidote for alcohol intoxication through the co-delivery of the nanocapsules of alcohol oxidase (AOx), catalase (CAT), and aldehyde dehydrogenase (ALDH) to the liver, where AOx and CAT catalyze the oxidation of alcohol to acetaldehyde, while ALDH catalyzes the oxidation of acetaldehyde to acetate. Administered to alcohol-intoxicated mice, the antidote rapidly accumulates in the liver and enables a significant reduction of the blood alcohol concentration. Moreover, blood acetaldehyde concentration is maintained at an extremely low level, significantly contributing to liver protection. Such an antidote, which can eliminate alcohol and acetaldehyde simultaneously, holds great promise for the treatment of alcohol intoxication and poisoning.