Compound wooden chicken particle quality control and evaluation method based on anti-hepatitis biological activity

[0035](1) Preparation of the solution solution

[0036]Waterfillibin is dissolved in DMEM methionine, choline lacking culture solution (MCD), formulated into aqueous flying gliite culture, microporous filtration, to give a solution solution;

[0037](2) Preparation of the test solution

[0038]Repairing the wood chicken particles are added to 50% ethanol, and the ultrasonic treatment is cooled, filtered and willed with solvents, and the ointment is obtained, and the paste is calculated; the extract is dissolved in DMEM complete culture solution, formulated into a drug-containing culture medium, micro Hole filtering, can be used for test solution;

[0039](3) Establishment of hepatocyte injury model

[0040]The 6-well culture plate was taken, and the cultured AML12 hepatocyte suspension was administered. After the cells are attached to the cell, the original culture solution is discarded, and the MCD culture solution is added, and the cellular activity is continued. Cell activity is time-dependent ...

[0063]Compound Wood Granular Quality Control and Evaluation Method Based on Anti - Hepatitis Biological Activity, including the following steps:

[0064]1 instrument and test

[0065]1.1 instrument

[0066]HD2-BCN-1360B biochemical clean work table (Harbin Donglian Electronic Technology Development Co., Ltd.); Sunrise Refrigerator (Switzerland Tecan); - 80 ° C Ultra-low Water Refrigerator (Haier); NKSY series constant temperature water bath pot (Changzhou Nuo Base Instrument Co., Ltd.); US AutoFlow type CO2 incubator (German Nuaire); AE31 type inverted phase difference microscope (MATIC); CP225D Electronics Analysis Balance (Germany Sarto-Rius Analysis Tianping Co., Ltd., one million); kq5200b Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0067]1.2 test

[0068]Fetal Cattle Serum (Beijing Fort-Biotechnology Co., Ltd.); DMEM High Sugar medium (US GIBCO); dimethyl sulfoxide (SIGMA); trypsin (US GIBCO); cell proliferation and toxic test kit (CCK-8) (Dalian Meilun Biotechnology Co., ...