2 results about "Tumor marker" patented technology

The invention discloses screening and application of a nucleic acid aptamer specifically targeting a breast cancer cell line MCF-7. The nucleotide sequence of the nucleic acid aptamer refers to a DNAfragment represented by the following sequence: a sequence 9-A: 5'-AGGAGCACGACTCTGACGTAGGATCGAGACGAGGTACGTAT-3'. The screening employs a cell-SELEX technology and comprises the following steps: firstsynthesizing an initial library; using the breast cancer cell line MCF-7 as target cells, and conducting incubation with the library; and conducting positive screening, elution, PCR amplification andsingle-stranded DNA preparation. For breast cancer cell detection, the nucleic acid aptamer has the advantages of simple and convenient operation, low cost, short cycle, and high accuracy; and targetmolecules combined with the nucleic acid aptamer of the invention are used for research, and tumor markers can be obtained. An effective tool and means are provided for early diagnosis of breast cancer, breast cancer typing and other applications, and the nucleic acid aptamer can be used for early diagnosis of breast cancer, preparation of breast cancer diagnostic drugs and kits.

The invention belongs to the technical field of biology, and relates to application of a reagent for detecting TRIM45 expression level in preparation of a hepatocellular carcinoma diagnosis and treatment preparation. The invention finds that TRIM45 is related to occurrence and development of hepatocellular carcinoma for the first time. Through bioinformatics analysis, it is found that TRIM45 has a high AUC value in hepatocellular carcinoma patients, and it is prompted that TRIM45 serving as the diagnosis marker of hepatocellular carcinoma has high accuracy and specificity. According to mRNA-seq data and clinical data from a cancer genome map database, it is verified that the expression level of TRIM45 can affect survival of hepatocellular carcinoma patients, it is prompted that TRIM45 has correlation with hepatocellular carcinoma, research on pathogenesis of hepatocellular carcinoma will be further enriched, and the application of the TRIM45 to the hepatocellular carcinoma is promoted. And a novel tumor marker and a novel therapeutic target are provided for diagnosis and prognosis monitoring of hepatocellular carcinoma.