5 results about "Azide" patented technology

The invention relates to a novel PEG (polyethylene glycol) in-situ covalent grafted modified alginate/polycation microcapsule. The microcapsule is a hydrogel bead of alginate, or the inside of the microcapsule is filled with liquid or hydrogel of alginate and the outer surface is a polyelectrolyte composite hydrogel film which is formed by polycation and alginate; a covalent bond is formed on the microcapsule through a click chemical reaction between an alkylene group and an azide group on the alginate, and PEG is grafted to the surface of the alginate hydrogel bead or alginate/polycation/ alginate microcapsule in an in-situ covalent mode; and a finished product is applicable to embedding of bioactive substances and living cells. The microcapsule film not only keeps excellent biocompatibility but also takes good film strength into consideration, so as to guarantee the integrity of the film in the processes of tissue cell transplantation as well as cell culture and application.

The invention relates to the field of biological chemistry and particularly relates to a rapid bacterium detection method. The detection method comprises the following steps: mixing a detecting sample, Cu<2+>, alkynyl and AuNPs functionalized by azide group, incubating, and observing the change of the solution color to obtain the bacterium concentration. According to the detection method provided by the invention, the specificity is strong, and the sensitivity is high, and the bacterium detection technology established by the invention has the advantages of rapidness, simplicity in operation and visible result compared with a bacteria culture method and a PCR method.

The invention discloses an azidated Rosamine (Ros-N3) fluorescence inductor. A synthetic method for the Ros-N3 fluorescence inductor is simple and comprises the following steps: with N-alkyl-m-aminophenol and p-nitrobenzaldehyde as raw materials, successively carrying out nitro reduction and amino azidation so as to obtain Ros-N3. Ros-N3 can selectively recognize H2S in a mixed aqueous solution (wherein a volume ratio of DMF to PB is 6: 4); after addition of HS<->, an ultraviolet spectrum undergoes a blue shift of 8 nm, fluorescence undergoes photoinduced electron transfer (PET), and fluorescence quenching occurs; after addition of other ions and biological mercaptan molecules (Cys, GSH and Hcy), the ultraviolet spectrum and fluorescence are free of obvious changes; and a detection limit is 3.5 ppm. High-resolution mass spectrometry proves that a reaction mechanism is reduction of azide into an amino group by H2S. Moreover, Ros-N3 is successfully applied to an experiment for identification of H2S in MGC-803 cells, so real-time detection of endogenous H2S in in-vivo cells with the probe is made possible, and Ros-N3 has potential application value.

The invention discloses a preparation method of a nitrogen-containing heterocyclic compound. The preparation method comprises the following steps: reacting a solution containing an alkynyl compound, an iodide and an azide compound in the presence of a catalyst and a radical initiator at -20 DEG C to 50 DEG C under the protection of inactive gas, removing the solvent to obtain a substance after thereaction is finished, dissolving the substance in toluene, reacting at 110-130 DEG C, and separating to obtain the nitrogen-containing heterocyclic compound. The synthesis method is simple and convenient, raw materials are wide in source, cheap and easy to obtain, and compared with previous synthesis methods, the synthesis method is economical and wide in substrate range.