The invention provides a biological synthesis method of N-acetyl-5-methoxytryptamine and an application of the N-acetyl-5-methoxytryptamine. The N-acetyl-5-methoxytryptamine is synthesized by using all the
protein coding genes for synthesizing the N-acetyl-5-methoxytryptamine or the recombinant
genetic engineering bacteria capable of expressing and synthesizing all the
protein coding genes of the N-acetyl-5-methoxytryptamine. And separating the N-acetyl-5-methoxytryptamine from the
system to obtain the N-acetyl-5-methoxytryptamine. The invention also discloses a recombinant vector containing a series of
enzyme coding genes for synthesizing the N-acetyl-5-methoxytryptamine and a recombinant
gene engineering bacterium. A series of
enzyme coding genes for coding the synthesized N-acetyl-5-methoxytryptamine in a recombinant vector and a series of enzymes for synthesizing the N-acetyl-5-methoxytryptamine are expressed in a
fermentation culture mode, the N-acetyl-5-methoxytryptamine is synthesized, and the yield of the N-acetyl-5-methoxytryptamine is high when the N-acetyl-5-methoxytryptamine is synthesized. The process is easy to implement, conditions are easy to control, and industrial production is suitable.