The invention relates to a high-resistivity high-entropy alloy film and a preparation method thereof, and belongs to the field of multi-principal-element alloy film materials. The novel high-entropy alloy film component contains six elements of Fe, Co, Ni, Al, Mn and W elements with different proportions, and the atomic percentages are 25%-35%, 25%-35%, 10%-25%, 5%-15%, 5%-15% and 5%-10% respectively. The preparation method comprises the following steps of ultrasonically cleaning a substrate, preparing a composite target, carrying out vacuumizing on a vacuum chamber, filling the vacuum chamberwith argon, carrying out pre-sputtering, heating the substrate and carrying out sputtering. A prepared film sample is smooth in surface, uniform in thickness and fine in structure, and the structureis a single-phase BCC solid solution, and is free of phase separation or intermetallic compound generation. According to the preparation method, the novel magnetic high-entropy alloy film with high hardness and high resistivity can be prepared, the thickness of the novel magnetic high-entropy alloy film is 500-2000 nm, and the highest resistivity is about 1800 microhm . m.