The invention discloses a
bletilla striata seed low-temperature storage device and method. The device comprises a
liquid nitrogen tank; a base is arranged at the bottom of the interior of the
liquid nitrogen tank; a plurality of stand columns are vertically arranged on the edge of the surface of the base; a storage mechanism is installed at the top of the base; the storage mechanism comprises trays and screw rods, and the multiple trays are sequentially stacked on the base; positioning holes are formed in the edges of the trays; the screw rods sequentially penetrate through the trays in a threaded manner from top to bottom; and the top of the
liquid nitrogen tank is rotationally connected with a sealing cover, and an adjusting sliding rod penetrates through the center of the sealing coverin a sliding mode. The method comprises the steps of S1, sterilizing seeds; S2, preparing a
matrix solution; S3, filling the seeds; and S4, refrigerating the seeds. According to the invention, duringrefrigeration, the
cell activity of the seeds is reduced, so that the seeds can be stored for a long time; and matrix
layers wrapping the outer walls of the seeds can be solidified into
solid, and thematrix
layers can play a role in external protection during
refrigeration, so that the seeds are prevented from directly making contact with liquid
nitrogen to be frostbitten, and the
survival rate of the seeds is increased.