The invention relates to the technical field of optical
microscopic imaging, particularly to a coherent anti-Stokes
Raman scattering microscopic imaging system and a coherent anti-Stokes
Raman scattering microscopic imaging method. The coherent anti-Stokes
Raman scattering v imaging
system comprises an
optical fiber laser device, a light splitting unit, a
photon crystal optical fiber, a first PPLNcrystal, a second PPLN
crystal, a
delay unit, a beam combiner and a
spectrometer, wherein the
femtosecond pulse
laser outputted by the
optical fiber laser device is divided into a
first light beam and a second
light beam by the light splitting unit, the
first light beam and the second
light beam independently propagate, the
first light beam pumps the
photon crystal optical
fiber to output super-continuum laser as Stokes light, the second
light beam is converted into tunable
infrared laser by the first PPLN crystal, the tunable
infrared laser is subjected to frequency multiplication by the second PPLN crystal so as to be used as detection light, the Stokes light and the detection light are synchronized and are combined into a third light beam so as to act on a sample, the sample is subjected to coherent anti-Stokes Raman scattering, the CARS
signal emitted by the sample is collected by the
spectrometer, and microscopic imaging is performed.