Coherent anti-Stokes Raman scattering microscopic imaging system and coherent anti-Stokes Raman scattering v imaging method

The invention relates to the technical field of optical microscopic imaging, particularly to a coherent anti-Stokes Raman scattering microscopic imaging system and a coherent anti-Stokes Raman scattering microscopic imaging method. The coherent anti-Stokes Raman scattering v imaging system comprises an optical fiber laser device, a light splitting unit, a photon crystal optical fiber, a first PPLNcrystal, a second PPLN crystal, a delay unit, a beam combiner and a spectrometer, wherein the femtosecond pulse laser outputted by the optical fiber laser device is divided into a first light beam and a second light beam by the light splitting unit, the first light beam and the second light beam independently propagate, the first light beam pumps the photon crystal optical fiber to output super-continuum laser as Stokes light, the second light beam is converted into tunable infrared laser by the first PPLN crystal, the tunable infrared laser is subjected to frequency multiplication by the second PPLN crystal so as to be used as detection light, the Stokes light and the detection light are synchronized and are combined into a third light beam so as to act on a sample, the sample is subjected to coherent anti-Stokes Raman scattering, the CARS signal emitted by the sample is collected by the spectrometer, and microscopic imaging is performed.