Prunella plant extract, preparation method and use thereof

[0066] Example 1

[0067] Preparation method of total triterpene extract in Prunella vulgaris plant extract:

[0068] (1) 500g of Prunella vulgaris ears, plus 80% ethanol by weight percentage, extract three times by heating and refluxing, each time 3500ml, the time is 2, 1.5, 1 hour; combine the three extracts, and use the method of reduced pressure and concentration to recover the ethanol , Concentrate to about 500ml; let stand overnight at room temperature, filter, filter residue A and filtrate A are separately used, and filter residue A is about 12g after drying;

[0069] (2) Filter residue A is dissolved in about 100ml of ethanol with a concentration of 80% by weight, add 50g of 100 mesh diatomaceous earth to mix the sample for adsorption, evaporate to dryness under reduced pressure for column chromatography, elute with 500ml of petroleum ether, recover petroleum ether, and discard the residue , And then eluted with 500ml ethanol to collect the total triterpene eluate;

[0070] (

[0071] Example 2

[0072] Preparation method of total triterpene extract in Prunella vulgaris plant extract:

[0073] (1) 100g of Prunella vulgaris ears, with a weight percentage of 80% ethanol, were extracted three times by ultrasonic method, 700ml each time, and the time was 2, 1.5, and 1 hour. Combine the three extracts, and use the method of concentration under reduced pressure to recover the ethanol until there is no alcohol; let stand overnight at room temperature, filter, filter residue A and filtrate A are used separately, and filter residue A is about 2.5g after drying;

[0074] (2) The filter residue A is dissolved in about 20ml of ethanol with a concentration of 80% by weight, add 10g of 100 mesh diatomaceous earth to mix the sample for adsorption, evaporate the ethanol, evaporate to dryness under reduced pressure for column chromatography, eluting with 100ml of petroleum ether, and recover the petroleum Ether, discard the residue, and then eluted with 100ml ethanol to col

[0076] Example 3

[0077] Preparation method of total triterpene extract in Prunella vulgaris plant extract:

[0078] (1) 100g of Prunella vulgaris ears, plus 80% ethanol by weight percentage, extract three times by ultrasonic method, 700ml each time, the time is 2, 1.5, 1 hour; combine the three extracts, and use the method of reduced pressure concentration to recover the ethanol Until there is no alcohol smell; let stand overnight at room temperature, filter, filter residue A and filtrate A are used separately, and filter residue A is about 2.6g after drying;

[0079] (2) Filter residue A is dissolved in about 80ml of 100% ethanol by weight percentage, add 10g of 100-mesh diatomaceous earth to mix the sample for adsorption, evaporate the ethanol, evaporate to dryness under reduced pressure for column chromatography, eluted with 100ml of petroleum ether, and recover the petroleum Ether, discard the residue, and then eluted with 100ml ethanol to collect the total triterpene eluate;