Technology for solid phase synthesis of peptide by WANG strategy

[0037] 1. Synthesis of TP5 Acylethylamide H-Arg-Lys-Asp-Val-Tyr-NHeT (1)

[0038] ①Boc-Tyr-OCH 2 - Preparation of polystyrene resin

[0039] Weigh 10g (10mmol) of chloromethyl (also known as Merrifield) resin (substitution equivalent 1mmol / g, particle size 100-200 mesh, cross-linking degree 1%), and three times the number of moles of Boc-Tyr-OH.K 2 CO 3 and 1 / 10 mole of KI were blended in 150mL DMF. The suspension was rotated and reacted on a hot bath at 60° C. for 25 h, and the reaction suspension was transferred to a No. G2 sand plate filter tube. Use DMF at about 60°C for diafiltration 5 times, about 50 mL each time. This was followed by diafiltration with the following solvents (× times): 95% EtOH × 3, DMF × 3, 50% EtOH / H 2 O×3, DMF×3, absolute ethanol×3. After fully drying, take out the resin and place it under an infrared lamp at about 50°C, and air-dry it to constant weight. It weighs 12.33g, and the weight gain is 2.33g (theoretical weight gain should be 2.44