Preparation method of ascorbic acid oxidase electrochemical biosensing composite modified electrode

A technology for modifying electrodes and acid oxidase, which is applied in the field of biosensors, can solve problems such as poor biocompatibility, small detection range, and impact on accuracy, and achieve the effects of strong affinity, short response time, and wide linear range

Inactive Publication Date: 2012-06-20
JIANGXI SCI & TECH NORMAL UNIV
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Problems solved by technology

[0003] The conductive polymer enzyme electrode prepared by the traditional electrochemical method (the bioelectrode in which the enzyme is immobilized on the surface of the electrochemical electrode by the conductive polymer film) has poor biocompatibility and has certain toxicity to the enzyme (M.Gerard ,

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[0011] detailed description

[0012] A preparation method of ascorbate oxidase electrochemical biosensing composite modified electrode includes the following steps:

[0013] 1. Preparation of electrolyte solution: firstly mix 1.42 mg 3,4-ethylenedioxythiophene, 2.93 mg sodium lauryl sarcosinate, 1 mg weight percent 0.1 wt% carbon sodium meter tube dispersion, 2.36 mg 1- Ethyl-3-methylimidazole ethyl sulfate was ultrasonically dissolved in a total volume of 1 ml of deionized water, and finally mixed uniformly to obtain an electrolyte solution.

[0014] 2. Preparation of high-performance conductive poly(3,4-ethylenedioxythiophene) nano-film composite modified electrode: Blow nitrogen into the electrolyte solution for 20 minutes to remove dissolved oxygen in the solution, using a conventional three-electrode system, The poly(3,4-ethylenedioxythiophene) nano-film composite modified electrode was prepared in one step at 1.1 V constant potential polymerization for 60 seconds, and the whole

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Abstract

The invention relates to a preparation method of an ascorbic acid oxidase electrochemical biosensing composite modified electrode. The preparation method comprises the steps of: preparing an electrolyte solution from 3,4-ethylenedioxy thiophene, sodium lauroylsarcosine, carbon nanotubes and 1-ethyl-3-methylimidazolium ethyl sulfate, and carrying out 1.1V constant-potential polymerization in a three-electrode system for 60 seconds to prepare the conducting poly(3,4-ethylenedioxy thiophene) nano film composite modified electrode by one-step reaction; and then drip-coating ascorbic acid oxidase buffer on the surface of the composite modified electrode, and finally covering the enzyme layer with a mixed liquid of carbon nanotubes, Nafion and ionic liquid. The constructed composite modified electrode has the advantages of good biological electric catalysis activity to vitamin C, wide linear range, low detection potential, rapid current response, significant sensitivity, high repeatability and stability, good selectivity, high immobilized enzyme activity and strong affinity; and the preparation method has the characteristics of low cost, simple process, and simplicity and easiness in operation and the like.

Description

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Claims

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Application Information

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Owner JIANGXI SCI & TECH NORMAL UNIV
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