Coronary heart disease related gene i.e. cyp17a1 gene and reagent, preparation or kit for in vitro detection and application thereof

[0022] Example 1, a gene related to coronary heart disease is CYP17A1 gene, the nucleotide sequence of the gene has the sequence shown in SEQ ID NO: 1. The test sample containing the gene of CYP17A1 can be obtained from cells from the tester, such as cells from blood, urine, saliva, gastric juice, hair, biopsy and autopsy materials, preferably from blood.

[0023] Embodiment 2, a reagent for in vitro detection of coronary heart disease-related genes, the reagent is used to detect the polymorphism of CTP17A1 gene rs4919687 site, the reagent includes the following primers:

[0024] Upstream primer: 5' CCACTCTTGCCCTTACAC 3'

[0025] Downstream primer: 5'GCAGTCCTCAGCCTCTAC 3'

[0026] The so-called "gene polymorphism" refers to the difference in the nucleotide sequence of each individual gene among the population. Those of ordinary skill in the art know that the polymorphic site described in the present invention is a single nucleotide polymorphic (SNP) site, that is, a single nucleotide change in the genome sequence; a difference in nucleotide sequence It can be embodied at the DNA level or at the RNA level. Therefore, the coronary heart disease-related gene of the present invention can be embodied at the DNA level, RNA level, preferably at the DNA level, more preferably at the genomic DNA level.

[0027]There are currently two strat

[0029] Example 3, as a preference of the above example, the reagent is a reagent for combining polymerase chain reaction and restriction fragment length polymorphism analysis or a reagent for combining polymerase chain reaction and direct sequencing. The reagents for detecting the rs4919687 site in the kit are different according to different detection methods. For example, when the polymerase chain reaction and restriction fragment length polymorphism analysis are used to detect the rs4919687 polymorphic site, the kit contains Restriction enzymes and corresponding restriction enzymes may be included, for example kpnI restriction enzyme and corresponding restriction enzymes.