Method for observing separation of micro-nano scale protein crystals from solution

[0022] Example 1: Detect whether there are micro-nano scale lysozyme crystals precipitated from the crystallization solution

[0023] The first step: drop the crystallization solution; first, drop the lysozyme crystallization solution into the quartz pool (height 20mm, bottom thickness 4mm, pool wall thickness 13.8mm, internal thickness 3mm), seal it, and carry out the experiment under normal temperature conditions;

[0024] The second step: adjust the mutual extinction of the two polarizers: first, turn on the laser light source, and the natural light emitted by it passes through the first polarizer to become linearly polarized light; then, the polarized light passes through the quartz crystal pool to reach the second linear polarizer, It is found that the light intensity is 0.05mV, indicating that the two polarizers are not completely extinguished; adjust the second linear polarizer until the light intensity is 0.01mV;

[0025] Step 3: Observe whether there are crystals: before

[0026] Example 2: Detecting whether there are proteinase K crystals of micro-nano scale separated out from the crystallization solution

[0027] The first step: drop the crystallization solution; first, drop the lysozyme crystallization solution into the quartz pool (height 20mm, bottom thickness 4mm, pool wall thickness 9.8mm, internal thickness 2mm), seal it, and carry out the experiment under normal temperature conditions;

[0028] Step 2: Adjust the mutual extinction of the two polarizers: first, turn on the laser light source, and the laser light passes through the first polarizer to become linearly polarized light; then, the polarized light passes through the quartz crystal cell to reach the second linear polarizer, and the light intensity is 0.02mV, indicating that the two polarizers have been completely extinguished;

[0029] Step 3: Observe whether there are crystals: before crystals are precipitated, the laser passes through the linear polarizer-quartz pool-crystallizat

[0030] Embodiment 3: Detect whether there are micro-nano scale catalase crystals separated out from the crystallization solution

[0031] The first step: drop the crystallization solution; first, drop the catalase crystallization solution into the plexiglass and seal it; then place the beaker between two semiconductor refrigerators, precisely control the temperature of the experiment, and adjust the experimental temperature to 0 ℃.

[0032] The second step: adjust the mutual extinction of the two polarizers: first, turn on the laser light source, and the natural light emitted by it passes through the first polarizer to become linearly polarized light; then, the polarized light passes through the quartz crystal pool to reach the second linear polarizer, It is found that the light intensity is 0.1mV, indicating that the two polarizers are not completely extinguished;

[0033]Step 3: Observe whether there are crystals appearing: before crystals are precipitated, the laser passes th