Controlled surface topography for enhanced protein crystallization rates

a technology of enhanced protein and controlled surface topography, which is applied in the direction of crystal growth process, immunoglobulin, peptides, etc., can solve the problems of difficult to integrate porous materials in bulk form with current testing systems and equipment, and the process of obtaining a protein crystal can be costly and inefficient, so as to prevent uncontrolled evaporation

Inactive Publication Date: 2008-05-22
ALFRED UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Benefits of technology

The technical effect of this patented method prevents unwanted evaporations during production or storage of proteins due to their high water content compared with other substances like salts. This ensures stable quality products without compromising on its overall properties such as dissolving ability and crystal formation process.

Problems solved by technology

The technical problem addressed in this patents relates to improving the efficiency and accuracy of analyzing biological samples containing large amounts of molecules like amyloid or other small organic substances called prions. Current techniques involve extracting these molecular structures from larger sample volumes, but they have limitations due to their high complexity and difficulty in integrating them onto smaller devices used for further analysis purposes. Therefore there remains room for improvement in identifying and enhancing the structural properties of certain types of moleculature formed at low concentrations without compromising its stability over long periods under various environmental factors including temperature fluctuations.

Method used

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  • Controlled surface topography for enhanced protein crystallization rates
  • Controlled surface topography for enhanced protein crystallization rates

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Embodiment Construction

[0046]FIG. 1 is a partial schematic diagram of a preferred embodiment of the invention, showing a plurality of different types if colloidal particles deposited onto the bottom inner surface of a well within a micro-titer plate for protein crystallization thereon.

[0047]A plate 100 containing a plurality of wells, for example, 96 wells, is provided, and each column (or row) is coated by a unique coating material. For example, the wells 20 in column A are provided with a silica coating, whereas the wells 30 in column B are coated with zirconia. The column or rows can be coated so that alternating ones have different coatings or repeating patterns of the same coating materials.

[0048]As shown, the wells 40 and 50 in columns C and D are also coated with different coatings, such as alumina or other suitable coating materials described above. Using several different types of coatings on a single plate as shown here provides a format that allows for higher throughput screening of possible prote

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Abstract

A method for accelerating protein crystallization on a substrate is provided, including the steps of providing a coating layer comprising a colloidal solution containing inert particles on at least one discrete testing portion of a testing substrate to provide at least one coated portion, and drying the coated portion so that the coated portion has an enhanced surface topography defined by the characteristics of the coating layer. A supersaturated protein solution is applied to the coated portion, and the testing substrate is placed in an incubator for crystallization, and the growth rate of the protein crystals is accelerated during incubation due to the enhanced surface topography of the at least one coated portion. The testing substrate is evaluated to determine the degree of protein crystallization until crystallization in complete, and the protein crystals are subsequently removed from the testing substrate subjected to specific characterization testing.

Description

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Claims

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Application Information

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Owner ALFRED UNIVERSITY
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