Deep-sea fish skin collagen peptide

[0021] Example 1:

[0022] A deep-sea fish skin collagen peptide. The preparation method includes fish skin pretreatment, fish skin collagen extraction, fermentation broth preparation, fish skin collagen peptide preparation, and fish skin collagen peptide purification. The specific steps are as follows:

[0023] 1) Fish skin pretreatment: Take the cod skin as the raw material, wash the skin in ice water, cut it into pieces, then place it in a solution containing a degreasing agent, stir continuously at 180rpm for 36h, and control the material-liquid ratio The degreasing temperature is 1:20, and the degreasing temperature is 12°C. Then, the defatted fish skin is soaked in 0.05mol / L NaOH solution for 50 minutes. Finally, the soaked fish skin is repeatedly washed with water until it is neutral, and the water is drained for use. Pretreatment can remove the fat wrapped around the collagen fibers and non-collagenous proteins such as globulin and keratin, which is convenient for subsequent

[0033] Example 2:

[0034] A deep-sea fish skin collagen peptide. The preparation method includes fish skin pretreatment, fish skin collagen extraction, fermentation broth preparation, fish skin collagen peptide preparation, and fish skin collagen peptide purification. The specific steps are as follows:

[0035] 1) Fish skin pretreatment: take cod skin as raw material, wash and cut the fish skin in ice water, then place it in a mixture of isopropanol and ether with a volume ratio of 1:1.5 at a speed of 200 rpm Stir continuously for 40h, control the material-liquid ratio to 1:25, and the degreasing temperature is 12℃. Then, the defatted fish skin is soaked in 0.1mol / L NaOH solution for 50min, and finally the soaked fish skin is repeatedly washed with water until it is neutral. , Drain the water for later use;

[0036] 2) Extraction of fish skin collagen: swell the pretreated fish skin with 0.8 mol / L glacial acetic acid solution for 40 minutes, control the material-to-liquid ratio of

[0043] Example 3:

[0044] A deep-sea fish skin collagen peptide. The preparation method includes fish skin pretreatment, fish skin collagen extraction, fermentation broth preparation, fish skin collagen peptide preparation, and fish skin collagen peptide purification. The specific steps are as follows:

[0045] 1) Fish skin pretreatment: take cod skin as raw material, wash and cut the fish skin in ice water, then place it in a mixture of isopropanol and ether with a volume ratio of 1:1.2 at a speed of 180 rpm Stir continuously for 36 hours, control the material-liquid ratio to 1:23, and the degreasing temperature is 8°C. Then, the defatted fish skin is soaked in 0.08mol / L NaOH solution for 30 minutes, and finally the soaked fish skin is repeatedly washed with water until it is neutral. , Drain the water for later use;

[0046] 2) Extraction of fish skin collagen: swell the pretreated fish skin with 0.6 mol / L glacial acetic acid solution for 30 minutes, control the material-to-liqu