Method using thallus whole cells to catalyze arginine to convert into agmatine

[0037] Example 1: Optimization of the crystallization conditions of agmatine

[0038] Since the existing scientific research data has not yet found relevant information on the crystallization of agmatine, the present invention investigates the effects of various organic solvents on the crystallization of agmatine, including methanol, ethanol and the like. Although these organic solvents are beneficial to the crystallization of agmatine to a certain extent, considering the safety factors of agmatine in application, the present invention focuses on the influence of ethanol on the crystallization of agmatine. Because low temperature is conducive to the precipitation of agmatine, the temperature during the experiment was controlled at 4~15℃, the concentration of agmatine in the converted clear liquid was 200~300g / L, and it was concentrated in vacuum to 1 / of the original volume. 2. The final result shows that adding 55% ethanol to the concentrate is more conducive to the recovery of

[0040] Example 2: Method for catalyzing the conversion of arginine to agmatine using whole cells of bacteria

[0041] The method includes the following steps:

[0042] (1) Preparation of whole cell arginine decarboxylase concentrate

[0043] a. Construction of arginine decarboxylase genetically engineered bacteria

[0044] The genetically engineered bacteria uses Escherichia coli K12 as a host, pET28A as a vector, and adiA as a target gene. The nucleotide sequence of the adiA gene is shown in SEQ ID NO. 1, and can be constructed using conventional molecular cloning techniques.

[0045] b. Pilot fermentation culture of genetically engineered arginine decarboxylase bacteria

[0046] s 1 Take the engineered bacteria glycerin tube to inoculate the activated bacteria on the petri dish for 24 hours; store the petri dish in a refrigerator at 4°C; use an inoculation loop to dig out a loop of plate seeds under aseptic conditions and inoculate the seed culture Base (50mL / 500mL Erlenmeyer flask).

[0059] Example 3: Method for catalyzing the conversion of arginine to agmatine using whole cells of bacterial cells

[0060] The method includes the following steps:

[0061] (1) Preparation of whole cell arginine decarboxylase concentrate

[0062] a. Construction of arginine decarboxylase genetically engineered bacteria

[0063] The genetically engineered bacteria uses Escherichia coli K12 as a host, pET28A as a vector, and adiA as a target gene. The nucleotide sequence of the adiA gene is shown in SEQ ID NO. 1, and can be constructed using conventional molecular cloning techniques.

[0064] b. Pilot fermentation culture of genetically engineered arginine decarboxylase bacteria

[0065] s 1 Take the engineered bacteria glycerin tube to inoculate the activated bacteria on the petri dish for 24 hours; store the petri dish in a refrigerator at 4°C; use an inoculation loop to dig out a loop of plate seeds under aseptic conditions and inoculate the seed culture Base (50mL / 500mL Erlenmeyer fl