Multienzyme recombinant cell and method of synthesizing D-pantolactone through multienzyme cascade catalysis

[0067] Example 1 Acquisition of L-pantoic acid lactone dehydrogenase encoding gene of Amycobacterium methylophilus

[0068] Using the published oxidase encoding gene from Amycolatopsis methanolica (GenBank accession number AIJ22255.1), after codon optimization, artificial synthesis (provided by Suzhou Jinweizhi Biotechnology Co., Ltd.) Gene synthesis service) L-pantoate lactone dehydrogenase encoding gene, the nucleotide sequence is shown in SEQ ID NO:1.

[0069] Example 2 Saccharomyces cerevisiae D-ketopantolactone reductase encoding gene acquisition

[0070] Using the published reductase encoding gene from Saccharomyces cerevisiae (GenBank accession number is CAA98692.1), after codon optimization, artificial synthesis (Suzhou Jinweizhi Biotechnology Co., Ltd. provides gene synthesis services) D- Ketopantolactone reductase encoding gene, the nucleotide sequence is shown in SEQ ID NO:3.

[0071] Example 3 Obtaining Exiguobacterium Glucose Dehydrogenase Encoding Gene

[0072] Using the published dehydrogenase encoding gene from Exiguobacterium sibiricum (GenBank accession number is ACB59697.1), after codon optimization, artificial synthesis (Suzhou Jinweizhi Biotechnology Co., Ltd. provides gene synthesis services) The gene encoding Bacillus glucose dehydrogenase, the nucleotide sequence is shown in SEQ ID NO:5.