Application for VEGF

[0020] Example 1 Preparation of Granular Fat and Adipose Stem Cell "Particle" Mixed Filling

[0021] 1.1 Liposuction: The operating doctor fully communicates with the patient to draw out the area that needs to be filled. Try to be bilaterally symmetrical. If there is another plan for bilateral asymmetry, calculate the approximate total volume of fat needed. Take the inner or outer sides of both thighs as the donor area, use manual syringe 0.04% lidocaine for swelling and anesthesia (avoid too high and too fast pressure), use 2mm non-invasive liposuction needle and 20mL disposable screw syringe for liposuction, liposuction volume double the amount required.

[0022] 1.2 Preparation of granular fat and fat stem cell "particle" mixed filling: After filling the syringe, put the syringe upright and suspend it naturally, then only remove the water in the lower layer of the fat particles in the syringe (if there is little water, you can not exclude it), do not put the suction The lipo

[0025] Example 2 Initial clinical trial of multi-point, multi-tunnel wet CAL filling

[0026] 2.1 Inclusion criteria

[0027] By adopting multi-point and multi-tunnel methods, from 2017 to 2018, autologous fat grafting was used to fill the wet CAL temporal part in multiple layers. The patient had no other physical abnormalities and agreed to sign the informed consent.

[0028] 2.2 Materials and methods

[0029] 2.2.1 Blank control group: 15 cases, 1 male and 14 females, aged 18-49 years, average 32.3 years old. The operations were performed on the temporal part, or combined with autologous fat in other parts. Among the patients, there were 3 cases of facial traumatic soft tissue depression, 2 cases of facial congenital depression, and 10 cases of temporal depression caused by aging. HGF control group: 15 cases, 2 males and 13 females, aged 19-48 years, with an average of 33.5 years. The operations were performed on the temporal part, or combined with autologous fat in other p