Biosynthesis method of dendritic glucosyl nanoparticles

[0028] Example 1:

[0029] Weigh 10g β-cyclodextrin and dissolve it in 1000mL phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 100U in vitro polysaccharidase preparation (New Pullulanase: Hydrolase family 13, 1, 4-α-glucan branching enzyme = 1:1) Constant temperature reaction for 6 hours, after the reaction is completed, the enzyme activity is inactivated by heating, centrifuged, and the supernatant obtained is vacuum dried to obtain the target product. The average size of the dendritic glucosyl nanoparticles is 35nm and the absolute molecular weight is 4.2×10 6 g / mol, polydispersity coefficient 1.0, branch density 7.3%.

[0030] Example 2:

[0031] Weigh 10g of large-membered cyclodextrin and dissolve it in 50mL of phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 200U in vitro polysaccharidase preparation (cyclomaltodextrinase: 1 of hydrolase family 13 , 4-α-glucan branching enzyme = 10:1) Constant temperature reaction for 24 hours, after the reaction is completed, heat inactivation of the enzyme, centrifugal treatment, vacuum drying of the obtained supernatant to obtain the target product. The average size of dendritic glucosyl nanoparticles is 78nm, and the absolute molecular weight is 1.9×10 7 g / mol, polydispersity coefficient 1.2, branch density 6.7%.

[0032] Example 3:

[0033] Weigh 10g of γ-cyclodextrin and dissolve it in 250mL phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 50U of in vitro polysaccharidase preparation (maltose glucosylase: hydrolase family 57 1, 4-α-glucan branching enzyme=1:10) Constant temperature reaction for 2h, heating to inactivate the enzyme after the reaction, centrifugation treatment, vacuum drying treatment of the obtained supernatant to obtain the target product. The average size of the dendritic glucosyl nanoparticles is 41nm, and the absolute molecular weight is 8.9×10 5 g / mol, polydispersity coefficient 1.1, branch density 6.1%.