Application of nerol in preparation of drug for preventing and treating oropharyngeal candidiasis

[0022] Embodiment 1: Nerol has influence on the growth curve of Candida albicans

[0023] Dilute the fresh Candida albicans suspension with sterile SDB medium and adjust its concentration to 5 × 10 7 CFU / mL cell suspension, add different concentrations of nerol solution (prepared by sterile 0.1% Tween 80) to make the final concentration 0 μL / mL, 1.54 μL / mL and 6.16 μL / mL, vortex shake evenly Afterwards, they were placed on a constant temperature shaker at 37°C and shaken at 200rpm. Immediately after shaking, draw 100 μL of Candida albicans culture solution from each centrifuge tube, dilute it 200 times with 0.85% normal saline, and place it in an ultraviolet-visible spectrophotometer to detect the absorbance value at 620 nm (OD 620nm ). Then every hour, use the same method to detect OD 620nm . The experiment was repeated three times, with time as the abscissa and OD 620nm The growth curve of C. albicans is plotted for the ordinate.

[0024] Experimental results

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[0026] Example 2: The protective effect of nerol on oropharyngeal candidiasis

[0027] Nerol has a good protective effect on oropharyngeal candidiasis caused by Candida albicans.

[0028] 1. Experimental grouping

[0029] The mice were randomly divided into 5 groups after being caged, the WT group was the growth control group not infected with Candida albicans; the Ca group was the group infected with Candida albicans without administration; the 2.7mg / kg NEL group was the group infected with Candida albicans And give 2.7mg / kg nerolidol treatment group according to body weight every day; 10.8mg / kg NEL group is infected with Candida albicans and give 10.8mg / kg nerolidol treatment group according to body weight every day; 20mg / kg FCZ group is infected Candida albicans and give 20mg / kg fluconazole treatment group according to body weight every day.

[0030] 2. Model construction

[0031]Construction of immunosuppressive model: In this experiment, 6-week-old BALB / c (2