Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof
A compound microbial inoculum and soil-borne disease technology, applied in the field of microorganisms, can solve the problems of single disease control, poor comprehensive effect, unstable effect, etc., and achieve the effect of simple process, maintaining ecological balance, and improving control effect.
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[0053] In another specific embodiment of the present invention, a preparation method of the above-mentioned composite microbial inoculum is provided, and the preparation method includes:
[0054] Mixing the original bacterial powder of Paecilomyces lilacinus and Bacillus polymyxa evenly to obtain a composite original bacterial powder;
[0055] Mix the adsorption mixture and the composite original bacteria powder evenly to obtain a mixture of microorganisms;
[0056] The mixture for adsorbing microorganisms and the synergistic mixture are uniformly mixed to obtain the composite microbial agent.
[0057] In another specific embodiment of the present invention, the original bacteria powder of Paecilomyces lilacinus and Bacillus polymyxa are obtained by fermentation culture.
[0058] In another specific embodiment of the present invention, the application of the above-mentioned composite microbial inoculants in the prevention and control of soil-borne diseases and the promotion of plant growth
Example Embodiment
[0063] Example 1 Preparation method of original bacteria powder of Paecilomyces lilacinus and Bacillus polymyxa
[0064] The preparation method of described Paecilomyces lilacinus comprises the following steps:
[0065] Step 1. Cultivation of slant strains. The original strains are inoculated into the PDA slant, and the inoculated slant medium is placed in a 28°C incubator for 14 days to obtain the first batch of slant strains.
[0066] Step 2. Prepare secondary liquid strains, each 1L liquid medium contains: 3g NaNO 3 ,1g K 2 HPO 4 , 0.5gMgSO 4 ·7H 2 O, 0.5g KCl, 0.01g FeSO 4 , 30g sucrose and distilled water. Put the sterilized liquid culture medium into a 3L Erlenmeyer flask, and each bottle is filled with 0.7-0.8L liquid culture medium. Under aseptic conditions, each bottle is inoculated with one slant strain prepared in step 1. After inoculation The liquid culture medium is placed on a shaker for shaking culture for 24-48 hours, at a temperature of 28° C., and a rotation speed of
Example Embodiment
[0073] Example 2
[0074] The composite microbial agent for preventing and controlling soil-borne diseases in this embodiment is composed of the following parts by weight: 1.5 parts of Paecilomyces lilacinus, 1.0 part of Bacillus polymyxa, 4.2 parts of calcium lignosulfonate, and carboxymethyl 4.2 parts of sodium cellulose, 10.4 parts of tobacco foam, 8.4 parts of auxiliary powder, 6.0 parts of starch, 8.4 parts of diatomaceous earth, 14.5 parts of composite synergist, 11.2 parts of attapulgite, and 30.2 parts of water.
[0075] The compound synergist includes alginic acid, humic acid, and chitin, and the mass ratio of the three is 1:1.5:1.
[0076] In the preparation method of the composite microbial inoculum in this embodiment, the steps are as follows:
[0077] 1. The preparation of the original bacterial powder is the same as in Example 1;
[0078] 2. Mix and stir the original bacteria powder of Paecilomyces lilacinus and Bacillus polymyxa to obtain a composite original bacteria powde
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