Application of wheat TaPRR95-B protein or coding gene thereof in regulation of plant height

[0023] Example 1 Genotyping and plant height correlation analysis of TaPRR95-B gene in core germplasm materials

[0024] 1. Select wheat micro-core germplasm materials (105 bred varieties and 157 local varieties) distributed in different wheat regions in my country as excavation materials for polymorphic SNP sites.

[0025] 2. Exon capture to obtain SNPs of micro-core germplasm materials

[0026] The Roche exon capture kit (SeqCap Pure Capture Bead Kit, 06977952001, Roche) was used to perform library construction and sequencing on the DNA of 262 materials. For specific methods, please refer to the kit instruction manual.

[0027] 3. The whole genome sequence comparison was carried out by BWA software, the variation detection and genotyping were carried out by GATK software, and the genotype of the gene in 262 materials was obtained.

[0028] 4. Using the plant height phenotype data of 262 micro-core germplasm materials for three years and three points (in Luoyang, Henan Province

[0033] Example 2 PRR95-B gene mutant phenotype statistics

[0034] In order to verify that the function of the TaPRR95 gene is related to the plant height, this example uses the EMS mutagenesis library of tetraploid wheat to obtain a mutant of the B copy of the gene, and the specific information is shown in the following table:

[0035] Table 2 PRR95-B gene mutation information

[0036]

[0037]

[0038] The present embodiment contrasts the plant height of wild type and mutant, obtains as follows figure 2 The results shown: Compared with the wild-type Kronos plant height, the plant height of the mutant lines kronos1181 and kronos2031 was significantly reduced, indicating that the PRR95-B gene can significantly reduce the plant height of wheat.