Trichoderma harzianum, culture method, application and composite biological agent

[0034] Example 1 Sources and Screening of Bacillus Bacillus

[0035] (1) Separation and purification of rhizosphere bacteria

[0036] To the application of "Three Sampling Law", the three-point sampling method is collected from the signs of the village of Sanjiang Guannan Autonomous County, Huijiang, Guangxi Zhuang Autonomous Region, separated according to soil microorganisms conventional flat dilution. It is called about 10 g of the root fluid, and the shaking of a glass container containing 100 ml of sterile water is oscillated for 30 min, so that the soil particles are uniform, and after standing for 5 minutes, the supernatant portion of the soil suspension is taken 10 times gradient. Dilution, dilute to 10 -3 10 -4 10 -5 , Take 0.1 ml of the dilution to PDA plate (potato 200g, glucose 20g, H) 2 O 1L, agar 20g, pH 7), all plates were placed in a constant temperature incubator for 24 hours, and after the surface of the tablet surface, the colonies were subjected to the pyrophils in

[0039] Example 2 Method for Preparation of Trichoderma Harzianum JNHC

[0040] (1) Trichoderma Harzianum JNHC strain was seeded onto the PDA liquid medium, and the culture temperature was 25-30 ° C, the culture time was 50-72 h, and the culture time was 50-72 h. Seed liquid;

[0041](2) Seed the seed liquid obtained by step (1) into a liquid fermentation medium, fermentation of fermentation under 25-30 ° C, producing fermentation fluid;

[0042] (3) Precation of the fermentation broth (2) inoculated in a solid fermentation medium in a solid fermentation medium in a ratio of 20% to 30% mass percent, and the solid fermentation 72 is at 25-30 ° C, air humidity of 60% to 90%. -96H, dry below 40 ° C to less than 10%, pulverized, and packaged.

[0043] The step of activating the hhazroma harzianum JNHC strain prior to step (1):

[0044] The JNHC strain of Harzberma Harzianum was inoculated on the PDA medium, and 3-6 days were cultured at 25-30 ° C, and the activated strain was obtained.

[0054] Experimental Example 1 Harzish Merca Madrid JNHC Fungi Applied to Wollery

[0055] This test has a total of 2 processes. Each process is repeated 3 times, each repeated random selection 15 Wo citrus, a total of 6 communities, randomly arranged in each community, totaling 90 pigs.

[0056] Treatment 1 (control group): Apply 50g / tree / time for the noise of the mandarin.

[0057] Treatment 2 (Experimental Group): Apply 50 g / treacokiner of the trichoderma Harzianum, Harzi Mikin, and the spacing 20D is administered at a time of 10 g / treacokine.

[0058] 1, loblast content in the cold mandarin

[0059] Each cell is randomly selected, each of which is divided into upper, medium, and lower three parts, each site is randomly extract 5 leaves, using a hand-held chlorophyllometer (Konica Minolta SpaD-502PIUS) to each leaves 3 Different locations are measured, and then the measurement results are measured as the SPAD value of the leaf, and finally take all SPAD values ​​of