Electrochemical immunosensor as well as preparation method and application thereof

An immunosensor and electrochemical technology, applied in the field of electrochemical detection, can solve the problems of high professional technical requirements for operators, cumbersome norovirus process, and low sensitivity, so as to improve sensitivity and specificity, facilitate centralized processing, and improve sensitivity high effect

Pending Publication Date: 2021-08-06
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: existing methods for detecting norovirus have problems such as cumbersome process, long time-consuming, expensive equipment, high professional technical requirements for operators, low sensitivity, and high detection limit.

Method used

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  • Electrochemical immunosensor as well as preparation method and application thereof
  • Electrochemical immunosensor as well as preparation method and application thereof
  • Electrochemical immunosensor as well as preparation method and application thereof

Examples

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[0030] Example

[0031] Preparation method of electrochemical immune sensor, preparation process figure 1 As shown, the specific steps include:

[0032] (1) Activation of screen printing carbon electrode (SPCE): Pipette 100 μL of 0.2 M droplets dropped on the surface of the electrode with a pipette, activate the circulating voltammetry, the scan potential range is -1.5 to 1.5V, scan speed It is 0.1V / s, scanning 10 laps, and the circulation voltammetry is stable to indicate activation, and the deionized water is rinsed, and the room temperature is dry.

[0033] (2) Electrodeposition nano gold particles (AUNPS): A 150 μl of mass fraction is 0.15% by a pipette to activate the surface of the working electrode, performing constant potential deposition, deposition potential to -0.2. V, deposition is 2400s, deionized water is rinsed, and it is dry at room temperature;

[0034] (3) Fixture of protein A: PBS dilution of 10 μl of 20 μg / mL of protein A by a pipette in step (2) depositin

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Abstract

The invention discloses an electrochemical sensor as well as a preparation method and application thereof. The electrochemical sensor sequentially comprises an activated screen-printed carbon electrode, gold nanoparticles and protein A from bottom to top, norovirus capsid protein monoclonal antibodies are modified on the protein A, and non-specific sites on the protein A are closed sites. The preparation method comprises the following steps: activating the screen-printed carbon electrode, sequentially depositing the gold nanoparticles, modifying protein A, modifying a norovirus capsid protein monoclonal antibody, and closing a non-specific site to obtain the electrochemical sensor. When the electrochemical sensor disclosed by the invention is used for quantitatively detecting the human norovirus capsid protein, the linear detection range is 1-10ng/mL, and the detection limit is 0.635-1.0 ng/mL. The electrochemical immunosensor is convenient to operate, low in cost, high in sensitivity and suitable for rapidly detecting the norovirus on site.

Description

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Claims

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Application Information

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Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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