A SNP Molecular Marker Influencing Net Gross Rate of Alpine Merino Sheep and Its Application

A merino sheep and molecular marker technology, applied in the field of molecular genetics, can solve the problems of not establishing a molecular marker system for fine wool traits, not effectively mining and utilizing important genetic resources, etc., and achieving easy and shortened interpretation of results. Nurturing cycles, enhancing the effect of early selection

Active Publication Date: 2022-02-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
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  • Claims
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AI Technical Summary

Problems solved by technology

In recent years, fine-wool sheep genetics and breeding experts at home and abroad have been devoting themselves to the research of candidate genes or molecular markers controlling wool traits, but no molecula

Method used

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  • A SNP Molecular Marker Influencing Net Gross Rate of Alpine Merino Sheep and Its Application
  • A SNP Molecular Marker Influencing Net Gross Rate of Alpine Merino Sheep and Its Application
  • A SNP Molecular Marker Influencing Net Gross Rate of Alpine Merino Sheep and Its Application

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Experimental program
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Embodiment 1

[0037] Example 1 Correlation between different genotypes of Alpine Merino sheep and net wool rate

[0038] 1. Sample collection

[0039] The samples were collected from 116 Alpine Merino sheep blood samples collected by the Sheep Breeding Technology Promotion Station of Gansu Province. 5 mL of blood was collected from the veins of each sheep and placed in blood collection tubes added with EDTA-K2 anticoagulant. After the blood samples were collected, they were mixed quickly and placed in ice packs Temporarily stored in the sampling box, transported back to the laboratory and stored in a -20°C refrigerator for DNA extraction.

[0040] 2. Main reagents and instruments

[0041] EDTA-K2 vacuum blood collection tubes were purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; NanoDrop2000 spectrophotometer from American Thermo Fisher Scientific Company; DL2000 Marker, agarose, nuclei

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Abstract

The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the net wool rate of Alpine Merino sheep and its application. The SNP molecular marker is located at the T/C mutation at the 7370557th nucleotide site on chromosome 4 of the International Sheep Reference Genome Oar_v4.0 version. The present invention also relates to a specific primer pair for detecting the above-mentioned SNP molecular marker using PCR technology, a kit containing the primer pair and a detection method for nucleotide polymorphism. Compared with the traditional detection method, the technology has high accuracy and is It has the advantages of fast speed, low cost, and easy judgment of results. SNP locus detection is used to carry out early selection of alpine merino sheep wool rate traits, shorten the breeding cycle, speed up the breeding process, establish an early selection technology for alpine merino sheep wool rate traits, and reduce the number of alpine merino sheep. The breeding time of the excellent traits of net gross rate and the reduction of breeding cost have high application value.

Description

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Claims

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Application Information

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Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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