Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid
A technology of lactic acid bacteria bacteriocin and waste liquid, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve problems such as waste of resources, and achieve the effects of increasing utilization value, extending industrial chain and reducing production costs.
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[0050] Example 1 Production of Lactic Acid Bacteria Preparation by Submerged Fermentation of Corn Alcohol Waste Grains Liquid
[0051] 1. Preparation of the filtrate of corn alcohol waste grains
[0052] Collect fresh alcohol waste grains, centrifuge it in a horizontal centrifuge (WL450 produced by Sichuan Jiangbei Machinery Factory), separate solid and liquid, take the separated liquid and filter it through a plate and frame filter, and collect the filtrate, which is the alcohol waste grains filtrate.
[0053] 2. Process conditions of lactic acid bacteria fermentation
[0054] 1. Expansion process of lactic acid bacteria:
[0055] (1) Production strain:
[0056] Lactobacillus gasseri 1-7;
[0057] Lactobacillus crispatus 23-5;
[0058] Enterococcus faecalis Entercococcus faecalis 23-7;
[0059] Freeze-dried, preserved strains, provided by the Institute of Industrial Microbiology, Liaoning Normal University.
[0060] Proportion of liquid culture medium: add 0.5% yeast extract to the
Example Embodiment
[0070] Example 2 Production of lactic acid bacteria by submerged fermentation of corn alcohol waste grains
[0071] 1. Preparation of the filtrate of corn alcohol waste grains
[0072] Collect fresh alcohol waste grains, centrifuge it in a horizontal centrifuge (WL450 produced by Sichuan Jiangbei Machinery Factory), separate solid and liquid, take the separated liquid and filter it through a plate and frame filter, and collect the filtrate, which is the alcohol waste grains filtrate.
[0073] 2. Process conditions of lactic acid bacteria fermentation
[0074] 1. Expansion process of lactic acid bacteria:
[0075] (1) Production strain:
[0076] Lactococcus lactis. LN992;
[0077] Lactobacillus brevis;
[0078] Freeze-drying and preservation of bacteria were provided by the Institute of Industrial Microbiology, Liaoning Normal University.
[0079] (2) Liquid culture medium:
[0080] Add 0.5% yeast extract to the filtrate of corn alcohol spent grains; add 0.5% sucrose; PH5.5-6.0 15 pou
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