Method for producing 5-hydroxytryptophan

Examples 1

Screening of Bacterial Strains

[0031]Plate screening medium: 5 g / L beef extract, 10 g / L soy peptone, 5 g / L NaCl, and 15 g / L agar. Liquid screening medium: 20 g / L sucrose, 20 g / L bean pulp, 3 g / L (NH4)2SO4, 5 g / L K2HPO4.3H2O, 1 g / L KH2PO4, 2 g / L beef extract, 4 g / L soy peptone, 5 g / L NaCl, 8 g / L tryptophan; pH is 7.0.

[0032]More than 100 soil or water samples were collected from Nanjing and surrounding areas to prepare appropriate concentrations of dilutions, the prepared samples are directly applied to screening medium plates, cultured at 37° C. for 1 day. Then, single colony of different morphologies are selected and inoculated into test tubes containing liquid medium. After shake culture at 30° C. and 200rpm for 2 days, fermentation supernatant is centrifuged. The 5-HTP content in the supernatant sis determined. 5-HTP production is detected in 4 of more than 500 colonies.

[0033]The strains obtained after initial screening are separately inoculated int

Examples 2

Identification of Strain JSC-69

[0035]The morphological, physiological and biochemical characteristics of JSC-69 are determined according to “Bergey's Manual of Determinative Bacteriology” (2nd Edition 2, 2004). After streak culture on plate medium for 1 day, the colonies are characterized by approximately round shape, milky white color, dark surface, opaque, irregular edge, coarse, sticky and spreading appearance. The colonies tightly adhere to the culture medium and are difficult to pick; in liquid culture, it has membrane without turbidity or precipitation. Individual bacillus should be 0.6×2.0 μm, this Gram-positive and aerobic bacterium is able to move without capsule or chain. The elliptical or cylindrical spore is 0.9×1.5 μm sitting in the middle or at one end with enlarged middle portion. The physiological and biochemical properties JSC-69 are determined, the results should be: the results show that this strain is catalase test positive. Gelatine liquefication, amyloly

Examples 3

Fermentation Culturing of Strain JSC-69 and Producing the Target Product

[0037]1. Culturing[0038](1) Primary seed culture: Monoclonal strains that have been freshly cultured in plate seed culture medium for ˜18 h are inoculated into 6 ml of liquid seed culture medium and cultured at 37° C. 225 rpm for ˜24 h. Plate seed medium composition ( / L): 5 g beef extract, 10 g soy peptone, 5 g NaCl, 1 L water and 15 g agar. Liquid seed medium composition ( / L): 5 g beef extract, 10 g soy peptone, 5 g NaCl and 1 L water, pH is7.0. Sterilization is carried out under 0.1 MPa for 20 min.[0039](2) Secondary seed culture: 6m1 of the primary seed culture medium is inoculated into a 1000 ml shake flask containing 200 ml of secondary seed culture medium and cultured at 30° C. 225 rpm for ˜24 h. OD600 is 6.0-10. Secondary seed culture medium composition is: 0.1% peptone, 0.2% corn syrup, 0.5% glucose and 0.5% yeast extract, pH is 8.0.[0040](3) Culture in fermentation tank[0041]1)Fermentation me