4 results about "Fluorescent microspheres" patented technology

The invention discloses a human Lp-PLA2 biotin-streptavidin fluorescence immunochromatographic assay card and a preparation method thereof and aims at providing an assay card which is supportive of quick combining, free of outside interference during reaction and high in specificity and stability and a preparation method thereof. The technical scheme includes that assay test paper comprises a substrate, a sample pad, a mark pad, a coated film and an absorption pad are connected on the substrate. The assay card is characterized in that an anti-Lp-PLA2 monoclonal antibody 1 of fluorescent microspheres and a biotin anti-Lp-PLA2 monoclonal antibody 2 are coupled on the mark, a streptavidin assay line T and a goat-anti-mouse polyclonal antibody quality control line C are parallelly arranged on the coated film. The invention belongs to the field of fluorescence immunochromatographic assay.

The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures. The methods of the invention possess the ability to multiplex, minimize the amount of biological sample, and have enhanced sensitivity and specificity toward target antibodies as compared with classical ELISA or Radio-Immunoprecipitation assays.

The invention discloses a preparation method and application of a fluorescent reagent strip for quantitatively detecting the concentration of ProAKAP4, and belongs to the field of in-vitro diagnostic reagents; a fluorescence immunochromatography technology is introduced into the detection of proAKAP4, and the prepared immunochromatography test strip for proAKAP4 adopts a double-antibody sandwich method; a proAKAP4 antigen in a sample pad is combined with a proAKAP4 antibody marked by fluorescent microspheres on a marking pad under the chromatographic action to form a compound, the compound moves to a detection line of a coating film under the chromatographic action, and the detection line of the coating film is coated with an antibody for identifying the other epitope of the proAKAP4 antigen to form a double-antibody sandwich compound; proAKAP4 with the concentration as low as 0.1 ng/mL in a seminal fluid sample can be detected, a detection result can be read through an immunofluorescence analyzer, professional operators are not needed, single-person quantitative detection of proAKAP4 is achieved, and the kit has the advantages of being convenient and rapid to use, easy to operate, high in sensitivity, easy to operate, low in detection cost and the like.