3 results about "Fluorescent pcr" patented technology

The invention relates to a kit, which designs a specific primer and a fluorescence labeled probe by adopting a real-time fluorescence polymerase chain reaction (PCR) technology and aiming at the conserved region of an NDM-1 pan-drug resistant gene and is used for specifically amplifying and detecting the NDM-1 pan-drug resistant gene segment. The technical scheme of the invention is to provide an NDM-1 pan-drug resistant gene PCR assay kit. The kit comprises a PCR reaction solution, wherein the PCR reaction solution comprises an upstream primer shown as SEQ ID NO.1, a downstream primer shown as SEQ ID NO.2 and a fluorescence probe shown as SEQ ID NO.3. The kit has high sensitivity and specificity, judges the result objectively and accurately, and provides a powerful tool for detecting existence of the NDM-1 pan-drug resistant gene in a clinical sample.

The invention relates to a real-time fluorescent PCR detection kit for bumblebee microsporidia and a detection method thereof, which are specially used for detecting bumblebee microsporidia. The kit comprises: (1) a positive standard; (2) negative control; (3) PCR reaction solution; (4) ExTaq enzyme; (5) sterile water. The invention has the following advantages: (1) good stability and specificity:detection of bumblebee microsporidia has high specificity, has no cross reaction with other microsporidia and bumblebee pathogens, and has good repeatability; (2) high sensitivity: the sensitivity can reach 20 copies/[mu] L; (3) easy and fast operation: the whole reaction can be completed within 2 hours. The kit can be used for detecting Nosema bombycis and differentiating and diagnosing the Nosema bombycis from other Nosema bombycis, and has important significance for early prevention and timely treatment of the disease. The kit can be used for detecting Nosema bombycis and differentiating and diagnosing the Nosema bombycis from other Nosema bombycis.