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3 results about "African swine fever virus" patented technology

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African swine fever virus (ASFV) is a large, double-stranded DNA virus in the Asfarviridae family. It is the causative agent of African swine fever (ASF). The virus causes a haemorrhagic fever with high mortality rates in domestic pigs; some isolates can cause death of animals as quickly as a week after infection. It persistently infects its natural hosts, warthogs, bushpigs, and soft ticks of the genus Ornithodoros, which likely act as a vector, with no disease signs. It does not cause disease in humans. ASFV is endemic to sub-Saharan Africa and exists in the wild through a cycle of infection between ticks and wild pigs, bushpigs, and warthogs. The disease was first described after European settlers brought pigs into areas endemic with ASFV, and as such, is an example of an emerging infectious disease.

Primer combination and kit for identifying African swine fever virus gene deletion strain and African swine fever epidemic strain through centrifugal microfluidic chip

InactiveCN113215311AStrong specificityMake up detectionMicrobiological testing/measurementDNA/RNA fragmentationAfrican swine feverEpidemiology

The invention belongs to the technical field of nucleic acid detection, and discloses a primer combination and a kit for identifying an African swine fever virus gene deletion strain and an African swine fever epidemic strain by using a centrifugal microfluidic chip. The kit comprises a microfluidic chip, an isothermal amplification premixed solution, an isothermal amplification enzyme solution and a positive standard. The invention further relates to a detection method adopting the kit. The detection method comprises the steps of coating of a primer composition, extraction of a nucleic acid sample to be detected, LAMP reaction and result interpretation. The detection system can rapidly, conveniently, efficiently, specifically and sensitively identify and diagnose African swine fever gene deletion strain and African swine fever wild strain under isothermal conditions, does not need expensive and complex instruments, provides a novel portable detection platform for epidemiological detection and prevention and control of African swine fever, greatly reduces detection cost, is easy to popularize in a large range, and has broad market prospects.

Primer combination and kit for identifying African swine fever virus gene deletion strain and African swine fever epidemic strain through centrifugal microfluidic chip

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Owner:SOUTH CHINA AGRI UNIV

Real-time fluorescent PCR primer and detection kit for A137R gene of African swine fever virus and application of real-time fluorescent PCR primer and detection kit

ActiveCN113981137AStrong specificityNo cross reactionMicrobiological testing/measurementMicroorganism based processesFluorescent pcrAfrican swine fever virus

The invention discloses a fluorescent quantitative PCR kit for detecting African swine fever virus and application. The fluorescent quantitative PCR kit contains primers as shown in SEQ ID NO.1 and SEQ ID NO.2, a fluorescent quantitative PCR reaction reagent, a standard substance and a reference substance. The fluorescent quantitative PCR kit disclosed by the invention can be used for detecting the African swine fever virus, is high in specificity, is only specifically combined with the African swine fever virus, and has no cross reaction with other swine viruses; the kit has the advantage of high detection sensitivity, has an excellent linear relationship in a copy range of 1 * 10 < 1 > to 1 * 10 < 8 > of a standard substance, can efficiently detect cotton swabs of African swine fever virus pigs and virus-carrying pigs without clinical symptoms, and improves the detection efficiency.

Owner:YANGZHOU UNIV

Test strip for detecting CD2v and MGF360 mucous membrane antibodies of African swine fever viruses and application of test strip

ActiveCN111912984AReduce the differenceImprove uniformityBiological testingImmunoassaysNitrocelluloseAfrican swine fever

The invention provides a test strip for detecting African swine fever virus CD2v and MGF360 mucous membrane antibodies and application of the test strip, and belongs to the field of production and technology of veterinary biological diagnosis products. The test strip comprises a nitrocellulose membrane provided with a detection line and a quality control line, wherein the detection line is coatedwith a mouse anti-swine SC protein monoclonal antibody; the quality control line is coated with purified protein of positive serum of African swine fever. the sample diluent contains quantum dot microsphere labeled recombinant protein MGF360 and recombinant protein CD2v; the sequence of the recombinant protein MGF360 is as shown in SEQ ID NO:4; the sequence of the recombinant protein CD2v is shownas SEQ ID NO:2. The test strip and the sample diluent are used for detecting the African swine fever virus CD2v and MGF360 mucous membrane antibodies, the test strip can be used for early detection of African swine fever infection, and have the advantages of strong specificity, high sensitivity and good stability.

Owner:JIANGSU ACAD OF AGRI SCI +1

3 results about "African swine fever virus" patented technology

Primer combination and kit for identifying African swine fever virus gene deletion strain and African swine fever epidemic strain through centrifugal microfluidic chip

Real-time fluorescent PCR primer and detection kit for A137R gene of African swine fever virus and application of real-time fluorescent PCR primer and detection kit

Test strip for detecting CD2v and MGF360 mucous membrane antibodies of African swine fever viruses and application of test strip

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