4 results about "Glutamine" patented technology

The invention relates to the technical field of cell culture, and in particular to a serum-free culture medium, a preparation method thereof and a culture method of mesenchymal stem cells. The culturemedium consists of the following components: non-essential amino acid, glutamine, hydrocortisone, dexamethasone, polyvinyl alcohol, recombinant human insulin, recombinant human transferrin, a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human Wnt-3a protein, a recombinant human fibronectin, recombinant human laminin, L-glutathione, L-ascorbic acid, beta-mercaptoethanol, ethanolamine, Pluronic F-68, Tween 80, cholesterol, adenine, sodium selenite and a basic culture medium. According to the invention, polyvinyl alcohol can replaceserum albumin in a serum-free culture medium in carrier function, so that proliferation capacity of mesenchymal stem cells is effectively improved. Polyvinyl alcohol has more stable performance thanserum albumin, and the problem that proliferation capacity and passage capacity of stem cells are reduced can be effectively solved.

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

The invention relates to a food and a preparing method thereof, in particular to multi-vitamin fruit vinegar and a preparing method thereof. The multi-vitamin fruit vinegar uses grapes, hawthorns andplums as main raw materials and is prepared by adding water, strong wine, sugar, monosodium glutamates, honey and salt. The grapes, the hawthorns and the plums are used as the main raw materials of the multi-vitamin fruit vinegar. The grapes are neutral in nature, have a sweet and sour taste, act on the lungs, the spleen and the kidney channel, and have the functions of supplementing qi and bloodand enriching the livers and the kidneys. The hawthorns contain multiple vitamins, tartaric acid, crataegolic acid and the like, and also contain flavonoid, lactone, glucide and mineral materials suchas calcium, phosphorus, ferrum and the like. The plums have a sweet and sour taste, are cool in nature, can clear liver heat, engender liquid, clear the liver and disinhibit water. The plums have multiple amino acids such as glutamine, serine, glycin, proline and the like, are nutritious and have pharmacophagous double functions. The three fruits are combined to form a compound multi-vitamin green health food. Because no additive is added, multi-vitamin fruit vinegar is novel green drink and seasoning which are pure natural and have a unique taste.

The invention discloses a food containing rich asparagus protein and belongs to the field of food processing. A minced-fish product comprises the following components in parts by weight: 10 parts of fish meat, 6-10 parts of potato starch, 10-15 parts of asparagus protein, 3-6 parts of granulated sugar, 2-4 parts of salt, 0.2-0.4 part of transglutaminase, 0.5-1.0 part of carrageenan, 0.4-0.8 part of sodium bicarbonate, 0.4-0.8 part of composite phosphate, 5-10 parts of iced water and 0.7-1.0 part of seasoning liquid. The asparagus is crushed by using a masher, alkali extraction and acid precipitation are utilized for extracting the asparagus protein. The minced fish product containing rich asparagus protein is rich in nutrition, high in protein content, resistant in storage resistance, good in gelling property and good in mouthfeel, well maintains the freshness of raw fish, can provide stable-quality materials for the processing industry of the minced fish product and has wide market prospect.