9 results about "Microarray" patented technology

A making method of a composite type biological chip based on a photonic crystal comprises four steps which are preparation of hard substrates, preparation of photonic crystal thin membranes, connection of membranous substrates and surface treatment of the substrates. (1) the preparation of hard substrates: common hard substrates are cleaned by soapy water, washed by de-ionized water and dried, and amino-group or hydroxyl is decorated on the surfaces of the substrates; (2) the preparation of photonic crystal: the nano-microspheres such as specific monodisperse silicon dioxide or polystyrene and the like, which are different in size according to the positions of testing signals, are assembled on the surfaces of the treated substrates to form the photonic crystal thin membrane with the thickness of 20 to 30 microns (3) the connection of membranous substrates: the surface of the photonic crystal thin membrane is laid with a layer of the membranous substrate and the connection is fixed by utilizing the chemisorption or the mutual function between positive and negative charges; (4) the surface treatment of the substrates: by using the methods such chemisorption and the like, chemical groups which can be fixedly connected with biomolecules to be tested are decorated on the membranous layer parts of the surfaces of the composite substrates which are formed in the step (3).

The invention aims to provide a microfluidic chip system for culture and multiplication behavior research of marine microalgae. Inoculation, batch culture, semi-continuous chemostat culture of the marine microalgae and a marine microalgae population multiplication behavior online monitoring process are integrated on a functional chip to be completed by means of fabrication of a scale integration chip and flexible combination of multiple cell technologies on the chip. The microfluidic chip system consists of two elementary units, wherein the first elementary unit refers to the microfluidic chip for culture and multiplication behavior research of the marine microalgae, and the second elementary unit refers to microfluidic chip peripheral equipment for culture and multiplication behavior research of the marine microalgae. The microfluidic chip system has the advantages that the microfluidic chip system is high in throughput, simple and convenient to operate and low in cost and is a novel platform for marine microalgae culture, population multiplication research and correlational research fields of using microalgae as experimental subjects.

Provided in the present invention are a molecule for biomarking cancers and a test composition for screening cancers and a detection method thereof comprising designing a number of oligonucleotide primers or probes by analyzing specimens for methylated regions of targeted genes PAX1, ZNF582, SOX1 and NKX6-1 in physical examination, and then using the oligonucleotide probes to detect whether methylation exists in the target genes, and further judge the likelihood of the occurrence of cancer. The detection methods for methylation status include methylation-specific PCR, (MSP), quantitative methylation-specific PCR (QMSP), bisulfate sequencing (BS), microarrays, mass spectrometer, denaturing high-performance liquid chromatography (DHPLC), pyrosequencing, and enzyme-linked immunosorbent assay (ELISA).

The invention relates to a screening method of silkworm hemocyte specific expressed genes and a method for analyzing and finding a hemocyte specific expression regulation element through cloning of a protease O gene promoter. The hemocyte specific expression regulation element is manufactured through the following steps that 1, a hemocyte specific expression candidate gene protease O gene is screened out through silkworm tissue chip expression data, transcriptome data, a quantitive RT-PCR and other methods, and the silkworm hemocyte specific expression of the gene is verified successfully; 2, a related gene promoter is successfully cloned, and functional analysis is carried out; 3, a Bac-to-Bac rhabdovirus expression system is successfully improved, an effective hemocyte promoter detection system is built, the activity of the candidate gene promoter is detected at the cell and the individual level, and tissue specificity of the promoter is verified; 4, the silkworm hemocyte specific gene protease O gene promoter is obtained through the study, and it is analyzed and found that the promoter is provided with the tissue specificity regulation element in the section between the upstream -333 and -80 of a transcriptional start site. The specificity expression of the gene on hemocyte is controlled.

The invention discloses preparation and application of a probe liquid chip for hypersensitive detection of miRNA. The basic principle of the probe liquid chip is an optical biosensor based on local surface plasma resonance effect of metal nano-arrays. A probe for rapid and hypersensitive detection of miRNA is prepared by the steps as follows: S1, modification of an LNA probe sequence; S2, arrangement of the metal nano-arrays; S3, disulfide reduction of the LNA probe sequence; S4, anchoring of the LNA probe. According to the preparation and application of the probe for rapid and hypersensitivedetection of miRNA, the probe ha high detection sensitivity, the chip is recyclable, and the service life is long.

The invention relates to a kit of a peripheral blood free-nucleotide miRNAs-based ultrasensitive detection device and a detection method of the kit. The kit comprises 1) a microarray detection pool; 2) an external magnetic field; 3) premixed reaction solutions A and 4) premixed reaction solutions B. During detection of multiple targets to be detected, the premixed reaction solutions A for respectively and correspondingly detecting the targets to be detected are configured in parallel and are respectively marked as a premixed reaction solution A1 and a premixed reaction solution A2. The kit iscombined with biomolecular diagnosis, nano-magnetic microsphere enrichment and biochip technologies to develop a novel technique capable of directly and accurately detecting peripheral blood miRNAs related to occurrence, development and drug resistance of a malignant tumor without amplification at high sensitivity and high flux. The kit has an important value in the aspects of noninvasive detection of medical tumors, personalized medicine application, survival after chemotherapy and the like.

The present invention relates to a composition comprising a probe for detecting six representative causative viruses of acute enteritis (norovirus genogroup I and genogroup II, rotavirus, hepatitis A virus, coxsackievirus, astrovirus, and adenovirus), and a DNA microarray, a kit, and a detection method comprising the composition. The present invention is effective due to high specificity and sensitivity to viruses. In addition, since the causative viruses can simply be detected at low cost compared to conventional detection methods, without expensive diagnosis devices or specialists, the present invention may be effectively used as a method for diagnosing viruses causing acute enteritis.

The invention relates to a gene chip for detecting transgenic plants and products containing screened gene NOS, belonging to the technical field of biological engineering. The chip comprises a glass slide with an aldehyde group modified surface, a screened gene NOS probe coated on the glass slide in an array mode, and a positive control, a hybridization positive control, a positive quality control probe, a negative quality control probe and a blank control which are fixed by nucleic acid, wherein the sequence of the screened gene NOS probe is NH2-(CH2)6-O-Poly (dT)15-AAGCATGTAATAATTAACATGTAATGCATGACGTTATTTA. The gene chip for detection of the screened gene NOS of the transgenic plants and the products of the invention can carry out specific detection on the screened gene NOS of the transgenic plants and the products thereof and has high specificity.