Method for preparing resting cells in process of preparing ethanol by utilizing anaerobic fermentation of synthesis gas

[0032] to prepare Clostridium autoethanogenum Resting cells of DSM10061 are taken as an example, and the specific preparation method includes the following steps:

[0033] (1) Experimental strains Clostridium autoethanogenum DSM10061 was purchased from the German Culture Collection, and the purchased strains were activated and enriched and grown under anaerobic conditions in an anaerobic box; during the enrichment and growth process, samples were taken every 12 hours, and the samples were measured at OD with a UV spectrophotometer. 600 Measure at the wavelength, and proceed to the next step after the growth is stable; after about 96 hours of enrichment culture, the microbial cells enter the growth stable period, and the biomass is stable and no longer grows;

[0034] The activation of the strains uses an activation medium, and the activation medium is a liquid medium, taking 1L capacity as an example, the formula is as follows: NH 4 Cl 0.7~1.2g, NaCl 0.7~1.2g, MgCl 2 0.2~