Method for determining content of calycosin-7-glucoside in radix astragali seu hedysari by ASE-HPLC (Accelerated Solvent Extraction-High Performance Liquid Chromatography) method

[0027] 1. Instruments and reagents

[0028] 1.1 Instruments:

[0029] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), ThermoU3000 UHPLC liquid chromatograph

[0030] 1.2 Reagents:

[0031] Water: Class I water in accordance with GB / T 6682;

[0032] Methanol (CH 4 O): chromatographically pure (for liquid chromatography);

[0033] Acetic acid (CH 3 COOH): analytically pure.

[0034] 1.3 Materials

[0035] Astragalus (produced in Inner Mongolia, batch number 1511100; produced in Gansu, batch number 15081901; produced in Inner Mongolia, batch number 150718) were purchased from Wuzhou or Yulin medicinal material market, and were identified by Huang Heng, deputy director of Guangxi Wuzhou Food and Drug Inspection Institute.

[0036] The reference substance calycocetin glucoside was provided by the China Institute for the Control of Biological Products, batch number: 111920-201505.

[0037] 2. Method

[0038] 2.1 Preparation of reference...