High-selectivity aggregation induced chemiluminiscence probe for <1>O2 as well as preparation method and application of chemiluminiscence probe

The invention discloses a high-selectivity aggregation induced chemiluminiscence probe for <1>O2 as well as a preparation method and an application of the chemiluminiscence probe. The structural formula of the chemiluminiscence probe is shown in the description, and the chemical name is 6-(4-(1,2,2-triphenylethyl)phenyl)-2,3-dihydrophthalazine-1,4-dione (TPE-PH for short). Chemiluminiscence of theprobe is weak when a solution is formed by the probe in a good solvent such as tetrahydrofuran. In a tetrahydrofuran-water mixed solvent, when proportion of water is larger than 70% (V/V), chemiluminiscence produced when formed nano-aggregate is oxidized by <1>O2 is enhanced sharply with increase of the proportion of water in the mixed solvent, the probe has typical aggregation induced reinforcedchemiluminiscence characteristic, and emission wavelength peak is 485 nm. The TPE-PH nano-aggregate has quite high chemiluminiscence selectivity for <1>O2 under physiological conditions and can recognize <1>O2 on nanomole level, which proves that TPE-PH is a novel chemiluminiscence probe capable of recognizing and detecting <1>O2 with high selectivity and high sensitivity in a physiological system.