Bone repairing biological material prepared by using abalone shell, and preparation method thereof

[0033] Example 1

[0034] (1) Dissolve 1.225g collagen in deionized water, stir magnetically for 12 hours to fully dissolve it, and prepare it into a 2.45wt% collagen solution;

[0035] (2) Dissolve 0.3 g of carboxymethyl chitosan in deionized water, stir magnetically for 2-3 hours to fully dissolve it, and prepare a 2wt% aqueous solution of carboxymethyl chitosan as an aqueous solution;

[0036] (3) mixing the collagen solution obtained in step (1) with the carboxymethyl chitosan solution obtained in step (2) in a volume ratio of 1:1 to prepare an aqueous phase solution (continuous phase);

[0037] (4) Take 0.1125g of abalone shell particles, add them to the above-mentioned aqueous phase solution, ultrasonicate for 5-10min, and prepare a particle dispersion mixture of 0.75wt% as a continuous phase;

[0038](5) Dissolve 0.8 g of polylactic acid-glycolic acid in 20 mL of dichloromethane to prepare a 4wt% polylactic acid-glycolic acid solution, which is also a dispersed phase as a

[0041] Example 2

[0042] (1) Dissolve 1.225g collagen in deionized water, stir magnetically for 12 hours to fully dissolve it, and prepare it into a 2.45wt% collagen solution;

[0043] (2) Dissolve 0.3 g of carboxymethyl chitosan in deionized water, stir magnetically for 2-3 hours to fully dissolve it, and prepare a 2wt% aqueous solution of carboxymethyl chitosan as an aqueous solution;

[0044] (3) mixing the collagen solution obtained in step (1) with the carboxymethyl chitosan solution obtained in step (2) in a volume ratio of 1:1 to prepare an aqueous phase solution (continuous phase);

[0045] (4) Take 0.45g of abalone shell particles, add them to the above-mentioned aqueous phase solution, ultrasonicate for 5-10min, and prepare a particle dispersion mixture of 3.0wt% as a continuous phase;

[0046] (5) Dissolve 0.8 g of polylactic acid-glycolic acid in 20 mL of dichloromethane to prepare a 4wt% polylactic acid-glycolic acid solution, which is used as an oil phase and is a