Tissue culture and rapid propagation method of citrus limonum

[0032] Example one

[0033] (1) Explant collection: Select the middle and upper part of the perfume lemon plant that was born half wood and no obvious disease, and the sectioned stems with sufficient light as the explants. After collection, the water retention and moisturizing treatment were brought back to the laboratory.

[0034] (2) Explant induction: Rinse the explants collected in step (1) back to the laboratory under running water for 5 hours, place them in a clean bench and sterilize them in 75% ethanol solution for 1 minute, and rinse with sterile water for 5 hours. After the second time, use sterile filter paper to absorb the water, sterilize it in 0.5% sodium hypochlorite solution for 20 minutes, rinse with sterile water for 5 times, absorb the water with sterile filter paper, and cut into about 2.0-2.5cm section with segments And inoculated into the induction medium, cultured in the dark at 20°C for 25 days to induce the formation of adventitious buds, with an induction rat

[0043] Example two

[0044] (1) Explant collection: Select the middle and upper part of the perfume lemon plant that was born half wood and no obvious disease, and the sectioned stem with sufficient light as the explant. After collection, it should be treated with moisture and moisture and brought back to the laboratory in time.

[0045] (2) Explant induction: Rinse the explants collected in step (1) back to the laboratory under running water for 5 hours, place them in a clean bench and sterilize them in 75% ethanol solution for 1 minute, and rinse with sterile water for 5 hours. After the second time, use sterile filter paper to absorb the water, sterilize it in 0.5% sodium hypochlorite solution for 20 minutes, rinse with sterile water for 5 times, absorb the water with sterile filter paper, and cut into about 2.0-2.5cm section with segments And inoculated into the induction medium, placed in 20 ℃ full dark culture for 25 days to induce the formation of adventitious buds, the inducti

[0054] Example three

[0055] (1) Explant collection: Select the middle and upper part of the perfume lemon plant that was born half wood and no obvious disease, and the sectioned stem with sufficient light as the explant. After collection, it should be treated with moisture and moisture and brought back to the laboratory in time.

[0056] (2) Explant induction: Rinse the explants collected in step (1) back to the laboratory under running water for 5 hours, place them in a clean bench and sterilize them in 75% ethanol solution for 1 minute, and rinse with sterile water for 5 hours. After the second time, use sterile filter paper to absorb the water, sterilize it in 0.5% sodium hypochlorite solution for 20 minutes, rinse with sterile water for 5 times, absorb the water with sterile filter paper, and cut into about 2.0-2.5cm section with segments Inoculated into the induction medium, cultured in the dark at 20°C for 25 days to induce the formation of adventitious buds, with an induction