Tissue culture rapid propagation method for nervilis fordii corm

[0019] An example of the rapid propagation method of the blue skyflower corm tissue culture of the present invention, comprises the steps:

[0020] (1) Extraction and disinfection of explants: As the explants, the bulbs of the blue skyflower are sterilized. First, 3-5 drops of detergent are dripped into a beaker with 50ml of tap water, and the explants are put into the beaker and stirred gently. 5min, then use cotton to clean the dirt on the surface of the explant, rinse with tap water for 20-30min; move the ultra-clean bench, soak in 75v / v% ethanol for 30s, rinse with sterile water, and then add 1-2 Sterilize 50ml of Tween-20 with 0.1v / v% mercuric chloride for 1-2min, rinse with sterile water for 3-5 times to obtain sterile explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure ;

[0021] (2) Acquisition of sterile rhizomes: Place the sterilized explants in the induction medium for adventitious bud induction, at a temperature ...

[0028] Another example of the rapid propagation method of the blue skyflower corm tissue culture of the present invention, comprises the steps:

[0029] Other conditions are the same as embodiment 1, only the following content is different:

[0030] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 of 6 - benzyl adenine, kinetin (KT) 0.3 mg L-1, naphthalene acetic acid 0.3 mg L-1, coconut milk 100 g L-1, medium pH value 6.8; corm induction coefficient 23.9.

[0031] In step (4), the transplanting survival rate was 93.8%.

[0033] Another example of the rapid propagation method of the blue skyflower bulb tissue culture of the present invention comprises the following steps:

[0034] Other conditions are the same as embodiment 1, only the following content is different:

[0035] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g·L-1 agar, 25g·L-1 sucrose, 1.5mg·L-1 of 6 - benzyl adenine, kinetin (KT) 0.5 mg L-1, naphthalene acetic acid 0.5 mg L-1, coconut milk 150 g L-1, medium pH value 6.8; corm induction coefficient 23.4.

[0036] In step (4), the transplanting survival rate was 91.6%.